Expression of RUNX3 protein in human gastric mucosa, intestinal metaplasia and carcinoma

European Journal of Clinical Investigation
Mitsuhiko OsakiHisao Ito

Abstract

Human runt-related transcription factor gene 3 (RUNX3) is considered as a possible candidate of tumour suppressor gene in human gastric carcinoma. To investigate the RUNX3 protein expression in human gastric mucosa and carcinoma, we generated a polyclonal antibody, AS251, which recognized amino acid sequences from 251 to 266 of human RUNX3. The AS251 antibody was immunoreactive with only RUNX3 protein, but not with RUNX1 and RUNX2. The AS251-antibody was available for Western blotting and immunohistochemistry using paraffin-embedded specimens. Western-blot analysis revealed that three (MKN-1, -7 and -45) of six human gastric carcinoma cell lines variably expressed RUNX3 protein, consistent with the expression pattern of RUNX3 mRNA reported previously by Li et al. (Cell 2002;109:113-24). Immunohistochemistry disclosed RUNX3 protein in most chief cells and a few gastrin-containing G cells in normal mucosa, but not in intestinal metaplasia and carcinoma cells. These data suggest that RUNX3 may play a physiologic role in chief cells and G cells in gastric mucosa, and that suppression of RUNX3 expression in intestinal metaplasia and carcinoma of human stomach may be implicated in gastric carcinogenesis.

References

Dec 1, 1992·Cancer Genetics and Cytogenetics·G Rege-CambrinG Saglio
Jul 3, 1991·Journal of the National Cancer Institute·J H KimA F Gazdar
Jan 1, 1986·Journal of Cancer Research and Clinical Oncology·H ItoE Tahara
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
Jul 6, 1995·The New England Journal of Medicine·C S Fuchs, R J Mayer
Dec 1, 1994·International Journal of Cancer. Journal International Du Cancer·H KuniyasuE Tahara
Oct 1, 1993·Trends in Genetics : TIG·H KagoshimaP Gergen
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·M C GhoziY Groner
Jan 1, 1997·Japanese Journal of Cancer Research : Gann·H HayashiH Ito
Apr 16, 1998·Nature·P GuilfordA E Reeve

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Citations

Nov 1, 2012·Seminars in Immunopathology·Tetsuya TsukamotoMasae Tatematsu
Sep 5, 2006·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Tetsuya TsukamotoMasae Tatematsu
Jul 6, 2010·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Sung Hoon JungIn-Sik Chung
Aug 11, 2005·Oncogene·Ralph CarvalhoMarian A J Weterman
Jan 27, 2010·The Journal of Biological Chemistry·Yun-Mi GohSuk-Chul Bae
Feb 14, 2009·Journal of Gastroenterology and Hepatology·Rita A Busuttil, Alex Boussioutas
Jul 6, 2007·Pathology International·Tetsuya TsukamotoMasae Tatematsu
Apr 4, 2006·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Toshiki HiramatsuHisao Ito
Apr 11, 2008·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·L Gutiérrez-González, N A Wright
Jul 31, 2007·Experimental Cell Research·Yasuo TanakaMasao Omata
Jun 4, 2008·Journal of Surgical Oncology·Kohei WakatsukiYoshiyuki Nakajima
Jan 27, 2011·Journal of Cellular Biochemistry·Yasusei KudoTakashi Takata
Sep 26, 2006·Best Practice & Research. Clinical Gastroenterology·Matti VauhkonenPentti Sipponen
Jul 5, 2016·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Eun Jung KimYun Kyung Lee
Mar 2, 2010·Journal of Korean Medical Science·Seung-Jin HongMun-Gan Rhyu
Feb 27, 2008·World Journal of Gastroenterology : WJG·Young-Seok ChoIn-Sik Chung
Jan 16, 2018·Annals of Surgical Treatment and Research·Eung Jin ShinMoo-Jun Baek

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