Expression of Sambucus nigra agglutinin (SNA-I') from elderberry bark in transgenic tobacco plants results in enhanced resistance to different insect species.

Transgenic Research
Shahnaz Shahidi-NoghabiGuy Smagghe

Abstract

Tobacco plants (Nicotiana tabacum cv Samsun NN) have been transformed with the gene encoding the type-2 ribosome-inactivating protein (RIP) SNA-I' from elderberry (Sambucus nigra) under the control of the Cauliflower Mosaic Virus 35S promoter. Previous research confirmed that these plants synthesize, correctly process and assemble a fully active RIP. Variability in protein expression was observed within the transgenic lines. The effects of the type-2 RIP SNA-I' delivered through a leaf feeding assay were evaluated in the laboratory on two economically important pest insects belonging to the orders of Hemiptera, the tobacco aphid (Myzus nicotianae) and Lepidoptera, the beet armyworm (Spodoptera exigua). In the experiment with aphids, significant effects were observed on the life parameters, such as survival, intrinsic rate of increase, net reproductive rate, mean generation time and mean daily offspring, whereas with caterpillars significant reduction in fresh weight as well as retardation in development were observed. In addition, significant increases in mortality were noted for insects fed on the transgenic lines as compared to wild type plants. This information provides further support for RIPs having a role in plant resista...Continue Reading

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Citations

Mar 25, 2014·Plant Science : an International Journal of Experimental Plant Biology·Bassam Al AtalahEls J M Van Damme
Aug 26, 2014·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Xiudao YuLanqin Xia
Mar 25, 2011·Phytochemistry·Gianni VandenborreEls J M Van Damme
Sep 10, 2010·Insect Biochemistry and Molecular Biology·Mohamad HamshouEls J M Van Damme
Jun 10, 2015·Phytochemistry·Liuyi Dang, Els J M Van Damme
Jan 22, 2011·Archives of Insect Biochemistry and Physiology·Shahnaz Shahidi-NoghabiGuy Smagghe
Aug 21, 2013·Molecular Ecology·Maria de la Paz Celorio-ManceraSören Nylin
Feb 13, 2010·Archives of Insect Biochemistry and Physiology·Katrien MichielsGuy Smagghe
Aug 31, 2013·Plant Science : an International Journal of Experimental Plant Biology·Peipei GuoXuewen Gao
Mar 7, 2013·Toxicon : Official Journal of the International Society on Toxinology·Fiorenzo Stirpe
Aug 29, 2014·Frontiers in Plant Science·Nausicaä Lannoo, Els J M Van Damme
Dec 16, 2016·Viruses·Surapathrudu Kanakala, Murad Ghanim
Jan 9, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Renata de Oliveira DiasOctavio Luiz Franco
Jan 30, 2018·Frontiers in Plant Science·Lore EggermontEls J M Van Damme
Jun 8, 2017·International Journal of Molecular Sciences·Sofie Van HolleEls J M Van Damme

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