Expression of SARS-CoV-2 Entry Factors in the Pancreas of Normal Organ Donors and Individuals with COVID-19.

Cell Metabolism
Irina KusmartsevaMark A Atkinson

Abstract

Diabetes is associated with increased mortality from severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Given literature suggesting a potential association between SARS-CoV-2 infection and diabetes induction, we examined pancreatic expression of angiotensin-converting enzyme 2 (ACE2), the key entry factor for SARS-CoV-2 infection. Specifically, we analyzed five public scRNA-seq pancreas datasets and performed fluorescence in situ hybridization, western blotting, and immunolocalization for ACE2 with extensive reagent validation on normal human pancreatic tissues across the lifespan, as well as those from coronavirus disease 2019 (COVID-19) cases. These in silico and ex vivo analyses demonstrated prominent expression of ACE2 in pancreatic ductal epithelium and microvasculature, but we found rare endocrine cell expression at the mRNA level. Pancreata from individuals with COVID-19 demonstrated multiple thrombotic lesions with SARS-CoV-2 nucleocapsid protein expression that was primarily limited to ducts. These results suggest SARS-CoV-2 infection of pancreatic endocrine cells, via ACE2, is an unlikely central pathogenic feature of COVID-19-related diabetes.

Citations

Jan 14, 2021·Journal of Biomedical Science·Ioannis P TrougakosMeletios A Dimopoulos
Jan 13, 2021·Nature Metabolism·Domenico Accili
Feb 5, 2021·Nature Metabolism·Janis A MüllerAlexander Kleger
Feb 6, 2021·Diabetes Technology & Therapeutics·Xiaofan JiaLiping Yu
Jan 3, 2021·Gastroenterology·Haizhou WangQiu Zhao
Apr 3, 2021·Frontiers in Immunology·Karthikeyan SubbarayanBarbara Seliger
May 11, 2021·Islets·Sarah IbrahimEmily K Sims
May 14, 2021·Frontiers in Endocrinology·Martha Campbell-Thompson, Shiue-Cheng Tang
May 21, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shuang HuaDewei Ye
Jun 4, 2021·Cell Metabolism·Chien-Ting WuPeter K Jackson
Jun 4, 2021·Cell Metabolism·Xuming TangShuibing Chen
May 27, 2021·Nature Metabolism·Laura MontefuscoPaolo Fiorina
Jun 12, 2021·Nature Communications·Charlotte SteenblockStefan R Bornstein
Jul 6, 2021·Frontiers in Endocrinology·Cyril P Landstra, Eelco J P de Koning
Jul 21, 2021·Frontiers in Cellular and Infection Microbiology·Syairah Hanan ShaharuddinDhruv Sareen
Jul 5, 2021·Diabetes Research and Clinical Practice·Sanjib SarkarPrasenjit Manna
Jul 2, 2021·Journal of Diabetes·Timothy P FosterDesmond A Schatz
Aug 14, 2021·Indian Journal of Endocrinology and Metabolism·S V Madhu, Nishant Raizada
Aug 11, 2021·Trends in Endocrinology and Metabolism : TEM·Shirin GeravandiAmin Ardestani
Jul 10, 2021·JCI Insight·Mirza Muhammad Fahd QadirFranck Mauvais-Jarvis
Aug 28, 2021·Frontiers in Endocrinology·Meghan Tahbaz, Eiji Yoshihara
Aug 24, 2021·Cell Reports Medicine·Amy L Clark, Raghavendra G Mirmira
Sep 2, 2021·Biosafety and Health·Rui-Ting Li, Cheng-Feng Qin
Aug 19, 2021·Endocrinology·Michael A KalwatMelanie H Cobb
Sep 17, 2021·Journal of Diabetes Investigation·Jun Shirakawa
Nov 6, 2021·Frontiers in Immunology·Collins N KhwatengeYongming Sang

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Datasets Mentioned

BETA
GSE84133
GSE81076
GSE85241
GSE86469
E-MTAB-5061

Methods Mentioned

BETA
scRNA-seq
smFISH
glycosylation
ELISA
Assay

Software Mentioned

Fluidigm
FindClusters
R
VlnPlot
DotPlot
RunTSNE
GraphPad Prism
HALO
CelSeq
GraphPad

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