Expression of select immune genes (surfactant proteins A and D, sheep beta defensin 1, and toll-like receptor 4) by respiratory epithelia is developmentally regulated in the preterm neonatal lamb

Developmental and Comparative Immunology
David K MeyerholzMark R Ackermann

Abstract

Preterm infants experience enhanced susceptibility and severity to respiratory syncytial virus (RSV) infection. Terminal airway epithelium is an important site of RSV infection and the extent of local innate immune gene expression is poorly understood. In this study, expression of surfactant proteins A and D (SP-AD), sheep beta defensin 1 (SBD1), and toll-like receptor 4 (TLR4) mRNA were determined in whole lung homogenates from lambs. SP-AD and TLR4 mRNA expression increased (p < 0.05) from late gestation to term birth. In addition, gene expression of LCM-retrieved type II pneumocytes (CD208+), adjacent epithelium (CD208-) and bronchial epithelium demonstrated that bronchiole-alveolar junction epithelium (combined CD208 +/-) had significant (p < 0.05) developmental increases in SP-AD, SBD1 and TLR4 mRNA, whereas CD208+ cells had statistically significant increases only with SP-A mRNA. Using immunofluorescence, SP-AD antigen distribution and intensity were also greater with developmental age. These studies show reduced SBD1, SP-AD, and TLR4 expression in the preterm lung and this may underlie enhanced RSV susceptibility.

References

Dec 1, 1981·The Anatomical Record·D G AlcornP M Robinson
Apr 1, 1995·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·E B BelknapJ C Baker
Feb 18, 1999·Pediatric Research·E MarinoniE V Cosmi
Jun 9, 2000·Annual Review of Physiology·A H Jobe, M Ikegami
Jan 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K R Khubchandani, J M Snyder
Jun 23, 2001·The New England Journal of Medicine·C B Hall
May 9, 2002·Respiratory Medicine·Y Aujard, B Fauroux
May 29, 2002·Pediatric Research·Machiko Ikegami, Alan H Jobe
Jun 26, 2002·Viral Immunology·Matthias Griese
Dec 10, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Suhas G KallapurCindy J Bachurski
Mar 21, 2003·Pediatric Research·Boris W KramerMachiko Ikegami
Jun 10, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·S J FlecknoeS B Hooper
May 15, 2004·The Journal of Infectious Diseases·Guy TalNinette Amariglio
Sep 14, 2004·Journal of Zhejiang University. Science·Hai-bo MouXiao-jian Meng
Nov 24, 2004·Microbes and Infection·David K MeyerholzMark R Ackermann
May 10, 2005·Pediatric Research·Elisabeth Förster-WaldlAndreas Spittler
Sep 6, 2005·Veterinary Immunology and Immunopathology·David K Meyerholz, Mark R Ackermann

❮ Previous
Next ❯

Citations

Feb 5, 2009·Journal of Veterinary Internal Medicine·U ChristmannR D Hite
Aug 11, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·David K MeyerholzMark R Ackermann
Dec 4, 2012·Viruses·Rachel J Derscheid, Mark R Ackermann
Aug 12, 2014·Pediatric Research·Augusto F SchmidtSuhas G Kallapur
Apr 19, 2011·Animal Reproduction Science·T R KannakiP C Verma
Apr 2, 2008·Developmental and Comparative Immunology·Shanjana AwasthiKevin M Brown
Aug 8, 2009·International Journal of Experimental Pathology·Alicia OlivierMark Ackermann
Jun 5, 2007·Immunobiology·Grith Lykke SorensenUffe Holmskov
Jul 1, 2008·Expert Review of Clinical Immunology·Mitchell R WhiteKevan L Hartshorn
Jul 23, 2013·Reproduction, Fertility, and Development·F NarciandiC O'Farrelly
Nov 14, 2013·Physiological Genomics·K G MeadeC O'Farrelly
Feb 15, 2019·Frontiers in Immunology·Kieran G Meade, Cliona O'Farrelly
Jan 19, 2017·Molecular Medicine Reports·Lingjie MaXiaoming Liu
May 26, 2021·Peptides·C E Valdez-MiramontesB Rivas-Santiago
Oct 7, 2021·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Maria L DiasFiona B McDonald

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.