Expression of senescence-associated beta-galactosidase (SA-beta-Gal) by human skin fibroblasts, effect of advanced glycation end-products and fucose or rhamnose-rich polysaccharides

Archives of Gerontology and Geriatrics
V RavelojaonaL Robert

Abstract

Expression by cells of the SA-beta-Gal was shown to be a reliable indicator of the switch mechanism used by cells to enter the senescent phenotype. We used this method in order to explore the variation of SA-beta-Gal-positive cells with passage number and time spent in culture. Both parameters produced an increase of SA-beta-Gal-positive cells. The addition of a Maillard-product (advanced glycation end-product=AGE) to the fibroblast cultures also increased SA-beta-Gal expression. Fucose- and rhamnose-rich oligo- and polysaccharides (FROPs and RROPs, respectively) provided a significant protection against this AGE-induced increase of SA-beta-Gal-positive cells. It is speculated that these processes might well play an important role in skin aging.

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Aug 8, 2006·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·V RavelojaonaL Robert
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Citations

Jul 18, 2009·Biogerontology·Alvaro Macieira-Coelho
Jan 9, 2016·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·G NikolakisC C Zouboulis
Jun 7, 2011·Pathologie-biologie·L RobertJ Labat-Robert
Sep 30, 2015·Pathologie-biologie·J Labat-Robert, L Robert
Mar 8, 2013·Dermato-endocrinology·Paraskevi Gkogkolou, Markus Böhm
Oct 30, 2016·International Journal of Biological Macromolecules·Abid HussainMohammad Zuber
Aug 24, 2017·Environmental Toxicology·Su-Ying WenWei-Wen Kuo
Apr 9, 2014·Journal of Diabetes and Metabolic Disorders·Amir ArastehAli Akbar Moosavi-Movahedi
Mar 16, 2013·Clinical Chemistry and Laboratory Medicine : CCLM·Ladislas Robert, Jacqueline Labat-Robert
Jun 19, 2013·Clinical Chemistry and Laboratory Medicine : CCLM·Hervé PageonDaniel Asselineau
Oct 3, 2018·Aging and Disease·Maxime FournetAlexis Desmoulière
Jan 16, 2021·Scientific Reports·Phu-Tho NguyenHuu-Thanh Nguyen
Mar 7, 2021·Experimental Gerontology·Sandra Maria BarbalhoUri Arian Princ Flato
Nov 6, 2009·Current Opinion in Supportive and Palliative Care

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