Expression of serine proteinase inhibitor PP5/TFPI-2/MSPI decreases the invasive potential of human choriocarcinoma cells in vitro and in vivo

Gynecologic Oncology
M JinYohei Miyagi

Abstract

PP5/TFPI-2/MSPI is a Kunitz-type serine proteinase inhibitor with broad inhibitory spectra, abundantly produced by placenta and detected in the blood of pregnant women. Expression of PP5/TFPI-2/MSPI is exclusively detected in syncytiotrophoblasts of placenta, but is barely detectable in choriocarcinoma cells, a trophoblast-derived malignant tumor. Chromosome 7, in which the PP5/TFPI-2/MSPI gene is localized, is frequently lost in various types of tumors. We attempted to elucidate the relation between PP5/TFPI-2/MSPI expression and the malignant properties of choriocarcinoma cells. Human choriocarcinoma cells, JAR, were transfected with either a human PP5/TFPI-2/MSPI expression vector or an empty vector, and stable clones were obtained. Messenger RNA expression, protein secretion/localization, growth rate, and plating efficiency were evaluated. In vitro migration and invasive activity were determined by transwell chamber experiments. In vivo tumor growth was evaluated by the subcutaneous injection of cells to nude mice and followed by histological examination. Expression of mRNA and protein of PP5/TFPI-2/MSPI were confirmed, and a high producing clone and a low producing clone were chosen for further analysis. The majority of se...Continue Reading

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Citations

Mar 10, 2009·Clinical & Experimental Metastasis·Sophie IochmannPascale Reverdiau
Apr 11, 2008·Apoptosis : an International Journal on Programmed Cell Death·Prakasha Kempaiah, Walter Kisiel
Mar 21, 2007·International Journal of Cancer. Journal International Du Cancer·Yoshimasa NobeyamaToshikazu Ushijima
Oct 27, 2004·Biochemical and Biophysical Research Communications·Desheng KongHouyan Song
Sep 24, 2015·Biomarkers in Cancer·Shiro Koizume, Yohei Miyagi
Nov 26, 2002·The Journal of Biological Chemistry·Christina KastMalcolm Whiteway
May 19, 2006·Cancer Research·Jürgen BeckerLothar Schweigerer
Mar 14, 2007·Molecular Cancer·Prakasha KempaiahWalter Kisiel

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