Expression of serotonin derivative synthetic genes on a single self-processing polypeptide and the production of serotonin derivatives in microbes

Applied Microbiology and Biotechnology
Munyoung ParkKyoungwhan Back

Abstract

The plant-specific serotonin derivatives feruloylserotonin (FS) and 4-coumaroylserotonin (CS) are synthesized by the enzymes 4-coumarate:coenzyme A ligase (4CL) and serotonin N-hydroxycinnamoyltransferase (SHT). To express these genes coordinately, SHT was fused in-frame with the self-processing FDMV 2A sequence followed by 4CL in a single open reading frame and introduced into Escherichia coli or Saccharomyces cerevisiae. The transgenes were abundantly expressed in both recombinant microbes, but functional expression was achieved only in yeast, with cleavage at the 2A sequence yielding monomeric SHT-2A and 4CL as judged by immunoblot and product analyses. In the presence of an exogenous supply of precursors such as serotonin and ferulic acid, the recombinant yeast synthesized 4.5 mg l(-1) FS in the medium while 0.02 mg l(-1) FS was produced in the cells. Time-course analysis indicated peak accumulation of FS at 24 h after induction, and this level was maintained until 96 h. The optimum precursor concentration was 2 mM. A series of serotonin derivatives was produced by adding various cinnamate derivative precursors with serotonin; 2.5 mg l(-1) caffeoylserotonin (CaS) and 1.4 mg l(-1) CS were produced, whereas no sinapoylseroton...Continue Reading

References

Dec 1, 1994·Archives of Biochemistry and Biophysics·K BackJ Chappell
Jan 1, 1997·The Journal of General Virology·M L DonnellyM D Ryan
Oct 21, 1998·The Plant Journal : for Cell and Molecular Biology·M SekiK Shinozaki
Apr 17, 1999·The Plant Journal : for Cell and Molecular Biology·C HalpinM D Ryan
Jul 27, 1999·The Plant Journal : for Cell and Molecular Biology·J EhltingE Kombrink
Mar 23, 2002·Science·Motoaki SekiKazuo Shinozaki
Dec 4, 2004·Biological & Pharmaceutical Bulletin·Jung Seop RohJae Kwan Hwang
Aug 4, 2006·Applied and Environmental Microbiology·Jules BeekwilderArnaud Bovy
Jan 24, 2008·Plant Foods for Human Nutrition·Kyungjin LeeKyoungwhan Back

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Citations

Mar 25, 2009·Applied Microbiology and Biotechnology·Kiyoon KangKyoungwhan Back
Oct 26, 2010·Applied Microbiology and Biotechnology·Aymerick EudesDominique Loqué
Dec 15, 2011·Molecular Biotechnology·Ieva KalnciemaAndris Zeltins
Dec 25, 2015·Fungal Genetics and Biology : FG & B·James M Wagner, Hal S Alper
Sep 23, 2008·Metabolic Engineering·Kiyoon Kang, Kyoungwhan Back
Mar 24, 2018·Microbial Cell Factories·Su Jin LeeJoong-Hoon Ahn
May 20, 2021·Journal of Experimental Botany·Stefano NegriFlavia Guzzo

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