PMID: 7545156Sep 1, 1995Paper

Expression of Shigella dysenteriae serotype 1 O-antigenic polysaccharide by Shigella flexneri aroD vaccine candidates and different S. flexneri serotypes

Journal of Bacteriology
I FältA A Lindberg

Abstract

The potential utility of Shigella flexneri aroD vaccine candidates for the development of bi- or multivalent vaccines has been explored by the introduction of the genetic determinants rfp and rfb for heterologous O antigen polysaccharide from Shigella dysenteriae serotype 1. The serotype Y vaccine strain SFL124 expressed the heterologous antigen qualitatively and quantitatively well, qualitatively in the sense of the O antigen polysaccharide being correctly linked to the S. flexneri lipopolysaccharide R3 core oligosaccharide and quantitatively in the sense that typical yields were obtained, with ratios of homologous to heterologous O antigen being 4:1 for one construct and 1:1 for another. Moreover, both polysaccharide chains were shown to be linked to position O-4 of the subterminal D-glucose residue of the R3 core. In contrast to the hybrid serotype Y SFL124 derivatives, analogous derivatives of serotype 2a vaccine strain SFL1070 did not elaborate a complete heterologous O antigen. Such derivatives, and analogous derivatives of rough, O antigen-negative mutants of SFL1070, formed instead a hybrid lipopolysaccharide molecule consisting of the S. flexneri lipid A R3 core with a single repeat unit of the S. dysenteriae type 1 O ...Continue Reading

References

Nov 2, 1978·European Journal of Biochemistry·L KenneE Romanowska
Oct 1, 1977·Infection and Immunity·S M Payne, R A Finkelstein
Aug 1, 1990·Molecular & General Genetics : MGG·D O'Callaghan, A Charbit
Jun 1, 1990·Microbial Pathogenesis·A A LindbergB A Stocker
Apr 1, 1988·Vaccine·A A LindbergF P Reinholt
Apr 1, 1981·European Journal of Biochemistry·P E JanssonR Wollin

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Citations

Oct 28, 2008·Biological Chemistry·Gábor Nagy, Tibor Pál
May 16, 2002·Infection and Immunity·Malabi M VenkatesanJoan Butterton
Oct 1, 1996·Current Opinion in Biotechnology·H Billman-Jacobe
Aug 13, 2010·Microbial Cell Factories·Julian IhssenLinda Thöny-Meyer

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