Expression of sialyl-Tn epitopes on beta1 integrin alters epithelial cell phenotype, proliferation and haptotaxis

Journal of Cell Science
Monique ClémentJacques Le Pendu

Abstract

Sialyl-Tn (STn) is a tumor-associated carbohydrate antigen overexpressed in various carcinomas. To obtain its expression, murine carcinoma cells were transfected with the cDNA encoding ST6GalNAc I, a glycosyltransferase that acts exclusively on O-glycans. Overexpression of this enzyme led to the expected expression of cell surface STn epitopes. Surprisingly, the transfectants (STn+ cells) presented dramatic morphological changes and altered behavior. These STn+ cells lost the epithelial appearance of parental cells, became larger, more elongated and presented disorganized actin stress fibers. Additionally, their proliferation was impaired and their ability to migrate on fibronectin and hyaluronic acid was severely reduced. By contrast their adhesion on fibronectin remained unchanged. The major glycoprotein carrying the STn epitope was shown to be the integrin beta1 subunit. Anti-STn antibodies could restore migration of STn+ cells on fibronectin. A constitutively active permeant form of RhoA (TAT-RhoA(Val-14)) also restored motility on fibronectin of STn+ cells as well as a parental STn-cellular phenotype. These observations indicate that overexpression of ST6GalNAc I leads to a major change of the O-glycosylation of the integr...Continue Reading

References

Jan 21, 1991·International Journal of Cancer. Journal International Du Cancer·R SteinM J Mattes
Oct 1, 1983·Clinical & Experimental Metastasis·P NanniG Prodi
Aug 1, 1995·The Journal of Experimental Medicine·S KatohP W Kincade
Jan 2, 1993·International Journal of Cancer. Journal International Du Cancer·T KawanoA Kobata
May 1, 1995·British Journal of Cancer·D W MilesI Filipe
Jan 26, 1996·International Journal of Cancer. Journal International Du Cancer·M VictorzonC Haglund
Jun 21, 1996·International Journal of Cancer. Journal International Du Cancer·J L WertherS Itzkowitz
Feb 7, 1998·Science·A Hall
Nov 18, 1998·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·S E BaldusH P Dienes
Dec 9, 1998·Immunological Investigations·P FernstenJ Winfield
Jan 9, 1999·Glycoconjugate Journal·I BrockhausenS H Itzkowitz
Dec 2, 1999·Biochimica Et Biophysica Acta·I Brockhausen
Apr 15, 2000·The Journal of Biological Chemistry·M A ChellaiahK A Hruska
Jun 29, 2000·Biochemical and Biophysical Research Communications·M KonoS Tsuji
Apr 12, 2001·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·J Le PenduM Clément
Sep 1, 2001·Biochimie·A Harduin-LepersP Delannoy
Jul 2, 2002·The Journal of Biological Chemistry·Alexis C SemelSusan L Bellis
Sep 6, 2002·The Journal of Cell Biology·R C Liddington, M H Ginsberg
Sep 18, 2002·Immunological Reviews·Nancy HoggPaula Stanley
Dec 31, 2002·Current Opinion in Structural Biology·Timothy A Springer
Oct 2, 2003·Current Opinion in Cell Biology·Angeliki Malliri, John G Collard

❮ Previous
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Citations

Jun 16, 2012·Cancer Metastasis Reviews·Matthew J SchultzSusan L Bellis
Jan 10, 2012·Clinical & Experimental Metastasis·Hidenori OzakiHisashi Narimatsu
Sep 23, 2011·The Journal of Biological Chemistry·Katrine Ter-Borch Gram SchjoldagerHenrik Clausen
May 3, 2008·Science·Scott T LaughlinCarolyn R Bertozzi
Jun 17, 2010·Breast Cancer Research : BCR·Aurélie CazetPhilippe Delannoy
Jul 17, 2007·PLoS Pathogens·Danelle S EtoMatthew A Mulvey
Mar 14, 2008·Journal of the American Society of Nephrology : JASN·Soundarapandian VijayakumarQais Al-Awqati
Jun 21, 2014·Biochimica Et Biophysica Acta·Fabio Dall'OlioMariella Chiricolo
May 26, 2010·The Journal of Cell Biology·David J GillFrederic Bard
Mar 20, 2012·Trends in Molecular Medicine·Shigeru TsuboiMinoru Fukuda
Jul 6, 2010·Autoimmunity Reviews·Kalina A NikolovaTchavdar L Vassilev
Jun 12, 2013·International Journal of Cancer. Journal International Du Cancer·Prakash RadhakrishnanMichael A Hollingsworth
May 30, 2015·Molecular Cancer·Bianca T HofmannGerrit Wolters-Eisfeld
Jul 8, 2014·The Biochemical Journal·Yoshinobu KariyaYasuhiro Hashimoto
Oct 17, 2014·Scientific Reports·Elena AgliariLuca Businaro
May 14, 2009·British Journal of Cancer·S JulienJ M Burchell
Jan 1, 2012·Biomolecules·Sylvain JulienPhilippe Delannoy
Mar 2, 2016·International Journal of Molecular Sciences·Jennifer Munkley
Apr 10, 2013·Molecular Oncology·José Alexandre FerreiraLúcio Lara Santos
Apr 8, 2020·Annals of Surgical Oncology·Hayato BabaTsutomu Fujii
Mar 15, 2019·Oncology Letters·Marina AlbertóValeria Inés Segatori
Jun 21, 2007·Molecular Cancer Research : MCR·John HuangMin-Chuan Huang
Aug 19, 2008·Molecular Cancer Research : MCR·Minyoung LeeYun-Sil Lee

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