Expression of signal transduction proteins during the differentiation of primary human erythroblasts

Journal of Cellular Physiology
Viviana di GiacomoGiovanni Migliaccio

Abstract

The high number (>10(8-10)) of primary human pro-erythroblasts (CD36high/CD235alow) obtainable in HEMA culture (Migliaccio et al., 2002) is exploited here to analyse the expression of proteins implicated in erythropoietin (EPO)-signalling (STATs, PI-3K, and PLCs) during the process of erythroid maturation. Human pro-erythroblasts progressed in 4 days of culture with EPO into basophilic- (CD36high/CD235amedium, 24 h), polychromatic-(CD36high/CD235ahigh, 48 h), and, finally, orthochromatic-(CD36low/CD235ahigh, 72-96 h) erythroblasts. During this maturation, STAT-1 was expressed up to the orthochromatic stage, expression of STAT-5, as well as of its target proteins BclxL and IRF1, remained constant up to 48 h (polychromatic-erythroblasts) but decreased by 96 h (orthochromatic-erythroblasts), while that of STAT-3 decreased constantly from 24 h on and became undetectable by 96 h. Expression of PI-3K rapidly decreased with differentiation since only 50% of original protein levels were detected by 48 h. On the other hand, among the members of PLC families investigated, PLC beta4 was not expressed, PLC beta2, delta1, and gamma2 were expressed at constant levels throughout the maturation process, while expression of PLC beta3 and of PLC...Continue Reading

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Citations

Sep 18, 2013·Advances in Biological Regulation·Yong Ryoul YangPann-Ghill Suh
Feb 16, 2008·The Journal of Biological Chemistry·Qin TongBarbara A Miller
Jul 25, 2015·Journal of Cellular Physiology·Maria Angela D'amicoAngela Di Baldassarre
Dec 24, 2011·International Journal of Cardiology·Darlington O OkonkoMyrtle Y Gordon
Dec 4, 2013·Advances in Biological Regulation·Matilde Y FolloLucio Cocco
Oct 13, 2012·Advances in Biological Regulation·Matilde Y FolloLucio Cocco
Dec 31, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Matilde Y FolloLucio Cocco
Mar 8, 2017·Human Gene Therapy Methods·Naoya UchidaJohn F Tisdale

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