Expression of simple epithelial cytokeratins in bovine pulmonary microvascular endothelial cells

Journal of Cellular Physiology
W F PattonD Shepro

Abstract

Polypeptides of bovine aortic, pulmonary artery, and pulmonary microvascular endothelial cells, as well as vascular smooth muscle cells and retinal pericytes were evaluated by two-dimensional gel electrophoresis. The principal cytoskeletal proteins in all of these cell types were actin, vimentin, tropomyosin, and tubulin. Cultured pulmonary microvascular endothelial cells also expressed 12 unique polypeptides including a 41 kd acidic type I and two isoforms of a 52 kd basic type II simple epithelial cytokeratin microvascular endothelial cell expression of the simple epithelial cytokeratins was maintained in cultured in the presence or absence of retinal-derived growth factor, and regardless of whether cells were cultured on gelatin, fibronectin, collagen I, collagen IV, laminin, basement membrane proteins, or plastic. Cytokeratin expression was maintained through at least 50 population doublings in culture. The expression of cytokeratins was found to be regulated by cell density. Pulmonary microvascular endothelial cells seeded at 2.5 X 10(5) cell/cm2 (confluent seeding) expressed 3.5 times more cytokeratins than cells seeded at 1.25 X 10(4) cells/cm2 (sparse seeding). Vimentin expression was not altered by cell density. By ind...Continue Reading

References

May 1, 1995·Histochemistry and Cell Biology·A M RickenP R Huber
Feb 1, 1993·Comparative Biochemistry and Physiology. Comparative Physiology·R Mineau-HanschkeD Shepro
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A B DodgeD Shepro
Dec 9, 1997·Journal of Clinical Pathology·M IwahashiR Nakano
Aug 1, 1996·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·A M RickenS Spanel-Borowski
Jan 11, 2011·Fertility and Sterility·Masaaki Iwahashi, Yasuteru Muragaki
Sep 1, 1994·Differentiation; Research in Biological Diversity·K Spanel-BorowskiW F Patton
Dec 24, 2005·Physiological Reviews·Dolly Mehta, Asrar B Malik
Jul 10, 2001·The Journal of Clinical Endocrinology and Metabolism·M IwahashiR Nakano
Mar 1, 2011·Experimental and Therapeutic Medicine·Masaaki IwahashiKazuhiko Ino

Citations

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 10, 1976·Biochemistry·G F Ames, K Nikaido
Sep 1, 1985·Cell·P M SteinertD R Roop
Sep 1, 1988·Cell and Tissue Research·C ViebahnF C Ramaekers
Jan 1, 1984·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·G A BlobelI Vogt-Moykopf
Jul 1, 1983·Microvascular Research·J D Gitlin, P A D'Amore

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