PMID: 9534887Apr 16, 1998Paper

Expression of skin-derived antileukoproteinase (SKALP) in reconstructed human epidermis and its value as a marker for skin irritation

Acta Dermato-venereologica
E BoelsmaM Ponec

Abstract

For the investigation of the skin irritancy potential of chemicals in an in vitro model, it is necessary to have sensitive end-points that predict the effects on native human skin. Our aim was to investigate whether the induction of the proteinase inhibitor SKALP in reconstructed epidermis can be used as a marker. The influence of culture conditions and the effect of topical application of sodium lauryl sulfate and oleic acid on SKALP expression were evaluated using immunohistochemistry and Northern blotting. SKALP expression was induced by serum, epidermal growth factor and fibroblasts. In the presence of retinoic acid and 1,25-dihydroxyvitamin D3 SKALP expression was inhibited, whereas supplementation with ascorbic acid and a-tocopherol had no effect. Tape-stripping of excised skin and topical treatment with sodium lauryl sulfate induced SKALP protein expression. Application of sodium lauryl sulfate and oleic acid on reconstructed epidermis also induced SKALP at the protein level but no significant effects could be demonstrated at mRNA levels. In conclusion, SKALP expression, which was increased upon application of sodium lauryl sulfate and oleic acid, can be used as an in vitro end-point for skin irritancy, irrespective of t...Continue Reading

Citations

Mar 30, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Thomas WelssKlaus R Schröder
Sep 1, 2000·International Journal of Pharmaceutics·M PonecM Mommaas
May 24, 2008·International Journal of Cosmetic Science·M PonecA Weerheim
Mar 18, 2003·International Journal of Cancer. Journal International Du Cancer·Joerg SchlingemannPeter Lichter
Jun 12, 2004·The Journal of Investigative Dermatology·Rita WongNicholas R Benson
May 14, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A El GhalbzouriM Ponec
Mar 27, 2018·Experimental Dermatology·Christian RiebelingTewes Tralau

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