Dec 12, 2012

Expression of small nucleolar RNAs in leukemic cells

Cellular Oncology (Dordrecht)
K J TeittinenOlli Lohi


Small nucleolar RNAs (snoRNAs) direct sequence-specific modifications to ribosomal RNA. We hypothesized that the expression of snoRNAs may be altered in leukemic cells. The expression of snoRNAs was analyzed in various leukemic cell lines by massive parallel sequencing (SOLiD). Quantitative real-time PCR (RT-qPCR) was used to validate the expression profiles. Our results show characteristic differences in the expression patterns of snoRNAs between cell lines representing the main subgroups of leukemia, AML, pre-B-ALL and T-ALL, respectively. In RT-qPCR analyses, several snoRNAs were found to be differentially expressed in T-ALL as compared to pre-B-ALL cell lines. snoRNAs are differentially expressed in various leukemic cell lines and could, therefore, be potentially useful in the classification of leukemia subgroups.

Mentioned in this Paper

Real-Time Polymerase Chain Reaction
Gene Expression Regulation, Neoplastic
Burkitt Lymphoma
Adult T-Cell Lymphoma/Leukemia
Ribosomal RNA
Reverse Transcriptase Polymerase Chain Reaction
Hairy Cell Leukemia
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
Jurkat Cells

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