Expression of SOD and APX genes positively regulates secondary cell wall biosynthesis and promotes plant growth and yield in Arabidopsis under salt stress

Plant Molecular Biology
Amrina ShafiAnil Kumar Singh

Abstract

Abiotic stresses cause accumulation of reactive oxygen species (ROS), such as hydrogen peroxide (H2O2) in plants. Sophisticated mechanisms are required to maintain optimum level of H2O2 that acts as signalling molecule regulating adaptive response to salt stress. CuZn-superoxide dismutase (CuZn-SOD) and ascorbate peroxidase (APX) constitute first line of defence against oxidative stress. In the present study, PaSOD and RaAPX genes from Potentilla atrosanguinea and Rheum australe, respectively were overexpressed individually as well as in combination in Arabidopsis thaliana. Interestingly, PaSOD and dual transgenic lines exhibit enhanced lignin deposition in their vascular bundles with altered S:G ratio under salt stress. RNA-seq analysis revealed that expression of PaSOD gene in single and dual transgenics positively regulates expression of lignin biosynthesis genes and transcription factors (NACs, MYBs, C3Hs and WRKY), leading to enhanced and ectopic deposition of lignin in vascular tissues with larger xylem fibres and alters S:G ratio, as well. In addition, transgenic plants exhibit growth promotion, higher biomass production and increased yield under salt stress as compared to wild type plants. Our results suggest that in du...Continue Reading

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Citations

Sep 14, 2016·Plant Physiology and Biochemistry : PPB·Hui YanDai-Fu Ma
Nov 23, 2017·Frontiers in Plant Science·Christophe LoixAnn Cuypers
Jan 25, 2018·International Journal of Molecular Sciences·Qingquan LiuLuqing Zheng
Jun 18, 2019·BioMed Research International·Xiulan XieYi Cai
Nov 30, 2018·Frontiers in Plant Science·Jessica SchmitzVeronica G Maurino
Jul 3, 2019·International Journal of Molecular Sciences·Sahar SellamiSylvie Dinant
Dec 27, 2019·Journal of Experimental Botany·Yongbin ZhouYouzhi Ma
Nov 13, 2020·Physiologia Plantarum·Anjali ShailaniAshwani Pareek
Feb 19, 2021·Frontiers in Plant Science·Petr DvořákTomáš Takáč
Apr 12, 2021·Nitric Oxide : Biology and Chemistry·Ming-Yue WeiHai-Lei Zheng

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