Expression of soluble versatile peroxidase of Bjerkandera adusta in Escherichia coli

Bioresource Technology
Martina MohorcicRoman Jerala

Abstract

Versatile peroxidase from white rot fungus Bjerkandera adusta was over-expressed in a soluble form in Escherichia coli. In the constructed enzyme model based on the selected gene from B. adusta, the active sites for oxidation of Mn(2+) ions and for oxidation of aromatic substrates were identified, both characteristic for versatile peroxidase. For over-expression of the recombinant enzyme different host strains, media formulations, growth temperatures, and fusion partners were tested. With the bacterial strain BL21(DE3)pLysS cultivated at 25 degrees C in auto-induction medium and presence of heme, a soluble peroxidase with incorporated heme and activity against different substrates was obtained. By exploiting an appropriate expression system and providing suitable culture conditions, the recombinant fungal peroxidases in soluble form can be produced in bacteria.

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Citations

Jun 8, 2011·Applied Biochemistry and Biotechnology·Chen Qi-HeSusanne Zibek
Sep 7, 2014·Biotechnology Advances·Katerina ZelenaRalf G Berger
Sep 14, 2011·New Biotechnology·Antonella AnastasiGiovanna Cristina Varese
Jan 1, 2010·Journal of Hazardous Materials·Antonella AnastasiGiovanna Cristina Varese
Feb 5, 2015·The FEBS Journal·Loredano PollegioniElena Rosini
Oct 11, 2011·The Biochemical Journal·Eva Garcia-RuizMiguel Alcalde
Jan 24, 2015·Bioprocess and Biosystems Engineering·Diana LinkeRalf G Berger
Apr 24, 2016·Applied Biochemistry and Biotechnology·Christoph J BehrensRalf G Berger
Aug 19, 2008·Bioresource Technology·Martina MohorcicRoman Jerala
Jan 15, 2010·Bioresource Technology·Antonella AnastasiGiovanna Cristina Varese
Oct 6, 2018·Chembiochem : a European Journal of Chemical Biology·Agathe BronikowskiVlada B Urlacher
Jan 24, 2021·Molecular Biotechnology·Alberto Moura Mendes LopesRosana Goldbeck

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