Abstract
We previously demonstrated that immunohistochemistry for γ-H2AX, a biomarker of DNA damage, is useful for early detection of urinary bladder carcinogens in rats. In a 28-day repeated-dose study, γ-H2AX was shown to have high sensitivity for detection of bladder carcinogens. However, no reports have evaluated whether a combination of multiple biomarkers may further improve sensitivity. Accordingly, in this study, we aimed to evaluate the applicability of bladder tissue and cancer stem cell markers, including cytokeratin 14 (KRT14), aldehyde dehydrogenase 1A1 (ALDH1A1), and cluster of differentiation 44 (CD44), as complementary markers for early detection of bladder carcinogens. Bladder samples obtained from male F344 rats orally treated with 14 bladder carcinogens and five nonbladder carcinogens for 28 days were used for immunohistochemical analysis of stem cell markers. In the bladder carcinogen-treated rats, increases in KRT14, ALDH1A1, and CD44 expression were observed in 9, 10, and 10 out of 14 groups, respectively, whereas the five nonbladder carcinogens did not cause upregulation of these markers. Although most epithelial cells with KRT14 or ALDH1A1 expression were also positive for CD44, KRT14 and ALDH1A1 expression were ...Continue Reading
References
Apr 1, 1983·Cancer Letters·Y MoriY Konishi
Mar 1, 1996·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·R D IrwinJ D Prejean
May 1, 1996·Journal of Applied Toxicology : JAT·R D IrwinJ K Haseman
Apr 16, 1998·The Journal of Biological Chemistry·E P RogakouW M Bonner
Aug 11, 2000·Chemico-biological Interactions·F Kassie, S Knasmüller
Apr 19, 2002·Nutrition and Cancer·K OgawaT Shirai
Aug 2, 2002·Carcinogenesis·Min WeiShoji Fukushima
Jun 28, 2005·Mutation Research·David KirklandLutz Müller
Jun 2, 2006·Critical Reviews in Toxicology·Samuel M CohenBarbara D Beck
Jun 6, 2009·Basic & Clinical Pharmacology & Toxicology·Kalavatala SaandeepGopabandhu Jena
Jan 30, 2010·International Journal of Toxicology·Christina L BurnettF Alan Andersen
Mar 21, 2012·Biochimica Et Biophysica Acta·Christophe E RedonWilliam M Bonner
Aug 15, 2012·Nature Reviews. Urology·Philip Levy HoKeith Syson Chan
Sep 27, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Frederick A BelandDaniel R Doerge
Aug 6, 2013·Journal of Toxicologic Pathology·Takeshi ToyodaKumiko Ogawa
Sep 5, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Takeshi ToyodaKumiko Ogawa
Oct 9, 2015·JAMA Oncology·Marcus G K CumberbatchJames W F Catto
Jan 6, 2016·International Journal of Molecular Sciences·Tomokazu OhishiToshiro Migita
Jun 21, 2016·Nature Communications·George PapafotiouApostolos Klinakis
Nov 5, 2016·Cancer Research·Pierre-Louis LoyherAlexandre Boissonnas
May 23, 2018·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Shigeki MotoyamaMasayuki Mishima
Sep 13, 2018·CA: a Cancer Journal for Clinicians·Freddie BrayAhmedin Jemal
Oct 12, 2018·Environmental and Molecular Mutagenesis·Ulla Plappert-HelbigHans-Jörg Martus
Jan 12, 2019·Regulatory Toxicology and Pharmacology : RTP·Samuel M CohenDouglas C Wolf
Feb 1, 2019·Journal of Applied Toxicology : JAT·Mizuki SoneKumiko Ogawa
Mar 24, 2020·Journal of Applied Toxicology : JAT·Takanori YamadaKumiko Ogawa
Nov 27, 2020·Journal of Toxicologic Pathology·Shugo SuzukiHideki Wanibuchi