Expression of stem cell markers on mononuclear cells derived from heparinized cadaveric organ donors before and after disconnection from the respirator

Transplantation Proceedings
B MachalinskiA Machalińska

Abstract

We have attempted to evaluate the level of the earliest human hematopoietic cell marker expression (CD34, CD117, CD133, CD184) on cells obtained from heparinized cadaveric organ donors before and after disconnection from the respirator. Moreover, we compared various cell populations: (1) coexpressing CD34/CD117; (2) CD34/CD133; (3) highly enriched hematopoietic stem cells (CD34+CXCR4+CD45+); and (4) highly enriched tissue-committed stem cells (CD34+CXCR4+CD45-). Finally, we analyzed whether the level of hematopoietic stem cell marker expression depended on the age of the donor. The expression of the membrane receptors (CD34, CD45, CD117, CD133, CD184) was studied by flow cytometry. We observed that the proportion of mononuclear cells expressing these markers slightly decreased in bone marrow harvested after disconnection from the respirator compared with the samples obtained before disconnection. Moreover, the proportion of cells expressing CD117 antigen depended on age of the donor.

References

Jul 17, 2003·The Journal of Clinical Investigation·Orit KolletTsvee Lapidot
Dec 31, 2003·Transplantation Proceedings·B MachalińskiM Ostrowski
Jan 29, 2005·Experimental Hematology·Rong YuanDavid E Harrison

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Citations

Oct 21, 2009·Current Eye Research·Anna MachalińskaBogusław Machaliński

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