PMID: 18201529Jan 19, 2008Paper

Expression of stromal cell-derived factor 1 is increased in the retina of experimental diabetic rats

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology
Yuan-Zhi YuanBei-Yan Tong

Abstract

To investigate the expression of stromal cell-derived factor 1 (SDF-1) in retina of experimental diabetic rats. Thirty four healthy male Wistar rats of six to eight weeks old were chosen and divided randomly into 3 groups: normal control (CON, n = 8), diabetes mellitus 2 months (DM2M, n = 14) and 3 months (DM3M, n = 12). Diabetes was induced by a single intraperitoneal injection of streptozotocin (STZ) (60 mg/kg). At the end point, immunochemistry and digital photomicrography analysis were employed to localize and quantitate the expression of SDF-1 in the retinas. The ratios of integrated optical density (IOD) to area of interest (AOI) were calculated to evaluate the intensities of positive staining. Values of z and t, as well as P value of each, were determined by Wilcoxon-Mann-Whitney test and Student's t-test, respectively. Immunofluorescent double-staining was used to indicate the co-expression of SDF-1 and hypoxia-inducible factor-1 (HIF-1), a pivotal transcription factor activated by hypoxia. The SDF-1 mRNA of the retina from DM2M (n = 4) and CON (n = 2) was detected by real-time RT-PCR. Copies of SDF-1 mRNA were then adjusted by the copies of beta-actin mRNA in each sample. Differences between groups were compared by a S...Continue Reading

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