Expression of T helper type 17 (Th17)-associated cytokines and toll-like receptor 4 and their correlation with Foxp3 positive cells in rectal biopsies of horses with clinical signs of inflammatory bowel disease

The Veterinary Journal
Karin M OlofssonRonny Lindberg

Abstract

Inflammatory bowel disease (IBD) in horses is an idiopathic disorder, encompassing different types of chronic intestinal inflammation. The pathogenesis of the disease remains to be established, but it has been suggested that an imbalance between regulatory T cells (Tregs) and T helper 17 (Th17)-associated cytokines and altered toll-like receptor 4 (TLR4) expression is associated with intestinal inflammation in other species. The aim of the present study was to quantify Tregs in rectal biopsies from horses affected with IBD by immunohistochemistry and to evaluate expression of genes encoding interleukin (IL)-12p40, IL-17A, IL-23p19 and TLR4 by real-time quantitative PCR. Rectal biopsies from 11 healthy horses and 11 horses with clinical signs of IBD, showing inflammation classified as chronic simple proctitis (CSP) or chronic active simple proctitis (CASP), were evaluated. Expression of IL-17A mRNA was greater in horses affected with CASP compared with horses with CSP or healthy horses. In contrast, expression of IL-12p40 was lower in horses with CSP compared with horses with CASP or healthy horses. TLR4 expression was greater in horses with CASP compared with healthy horses. A positive correlation was seen between the numbers o...Continue Reading

References

May 1, 1988·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H J GundersenA Vesterby
Apr 1, 1984·Journal of Comparative Pathology·R Lindberg
Sep 1, 1982·Veterinary Pathology·D A Pass, J R Bolton
Jun 1, 1996·The Journal of Experimental Medicine·F FossiezS Lebecque
Jun 20, 2002·The Journal of Biological Chemistry·Natalie J GartonIain C Sutcliffe
Sep 11, 2003·Clinical Chemistry and Laboratory Medicine : CCLM·Thomas WexPeter Malfertheiner
Nov 27, 2004·Research in Veterinary Science·A J DavidsonJ B Matthews
Dec 13, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Annerose BerndtN Edward Robinson
Sep 28, 2007·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Masayuki SarutaKonstantinos A Papadakis
Nov 28, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Lenka FrolovaHelena Tlaskalova-Hogenova
May 10, 2008·Journal of Veterinary Internal Medicine·I A BurgenerT W Jungi
Jul 8, 2009·The Veterinary Clinics of North America. Equine Practice·Karen A Kalck
Nov 6, 2009·Blood·Martin PelletierMarco A Cassatella
Aug 31, 2010·Inflammatory Bowel Diseases·Elke Cario
Mar 8, 2011·Inflammatory Bowel Diseases·Gheorghe HundorfeanJonas Mudter
Sep 1, 2008·Molecular Medicine Reports·Hiromitsu BanYoshihide Fujiyama
Jul 28, 2011·Journal of Digestive Diseases·Yi WangCheng Gong Yu
Mar 24, 2012·Veterinary Research·Johannes JungingerMarion Hewicker-Trautwein
Feb 13, 2013·Journal of Animal Science·B BaharT Sweeney
Apr 23, 2013·Clinical and Experimental Immunology·H ImaedaA Andoh
Jun 19, 2013·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Lequn Li, Vassiliki A Boussiotis
Aug 6, 2013·Veterinary Clinical Pathology·F CianJ Archer
Sep 18, 2013·International Reviews of Immunology·Edoardo TronconeGiovanni Monteleone

❮ Previous
Next ❯

Related Concepts

Related Feeds