Expression of tachykinin receptors inXenopus oocytes injected with poly (A)(+) RNA from cat dorsal root ganglion

Science in China. Series C, Life Sciences
H LiZ Q Zhao

Abstract

The expression of the types of tachykinin receptors in the dorsal root ganglion (DRG) neurons by means ofXenopus oocyte expressing system was studied. Poly(A)(+) RNAs were extracted from cat cervical and lumbar DRG. Two days after injection of Poly (A)(+) RNAs, the oocytes were recorded with the two-electrode voltage clamp technique. In the oocytes injected with DRG poly(A)(+) RNA, [Sar(9), Met(O(2))(11)]-substance P(Sar -SP, 1 mumol/L), neurokinin A (NKA, 1 mumol/L) or [beta-Ala(8)]-neurokinin A((4-10)) (Ala-NKA, 1 mumol/L) produced an inward current comprising a rapid spike and a long sustained oscillatory component for several minutes. Sar-SP induced response was blocked by NK-1 antagonist L-668, 169 (1 mumol/L), but not by NK-2 antagonist L-659, 877(1mumol/L). In contrast, Ala-NKA and NKA responses were only blocked by L-659, 877. The oocytes injected with DH Poly(A)(+)RNA also responded to Sar-SP and NKA with similar inward currents, which were selectively blocked by L-668, 169 and L-659, 877, respectively. These tachykinins-induced responses had a potent desensitization. The present data indicate expression of NK-1 and NK-2 receptors in DRG neurons, suggesting that there may be tachykinin autoreceptors on the nociceptive ...Continue Reading

References

Nov 13, 1990·European Journal of Biochemistry·K TsuchidaS Nakanishi
Jan 1, 1991·Annual Review of Neuroscience·S Nakanishi
Apr 1, 1993·Physiological Reviews·M Otsuka, K Yoshioka
Jan 1, 1996·Brain Research. Molecular Brain Research·T AndohY Kuraishi

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Citations

Feb 8, 2011·Journal of Neuroinflammation·Lynne ShanleyAlasdair MacKenzie
Sep 28, 1998·The European Journal of Neuroscience·H S Li, Z Q Zhao

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