Expression of TaWRKY44, a wheat WRKY gene, in transgenic tobacco confers multiple abiotic stress tolerances

Frontiers in Plant Science
Xiatian WangGuangyuan He

Abstract

The WRKY transcription factors have been reported to be involved in various plant physiological and biochemical processes. In this study, we successfully assembled 10 unigenes from expressed sequence tags (ESTs) of wheat and designated them as TaWRKY44-TaWRKY53, respectively. Among these genes, a subgroup I gene, TaWRKY44, was found to be upregulated by treatments with PEG6000, NaCl, 4°C, abscisic acid (ABA), H2O2 and gibberellin (GA). The TaWRKY44-GFP fusion protein was localized to the nucleus of onion epidermal cells, and TaWRKY44 was able to bind to the core DNA sequences of TTGACC and TTAACC in yeast. The N-terminal of TaWRKY44 showed transcriptional activation activity. Expression of TaWRKY44 in tobacco plants conferred drought and salt tolerance and transgenic tobacco exhibited a higher survival rate, relative water content (RWC), soluble sugar, proline and superoxide dismutase (SOD) content, as well as higher activities of catalase (CAT) and peroxidase (POD), but less ion leakage (IL), lower contents of malondialdehyde (MDA), and H2O2. In addition, expression of TaWRKY44 also increased the seed germination rate in the transgenic lines under osmotic stress conditions while exhibiting a lower H2O2 content and higher SOD, ...Continue Reading

References

Feb 1, 1992·Plant Molecular Biology·S Torres-SchumannJ A Pintor-Toro
Sep 15, 1998·Free Radical Research·K Moore, L J Roberts
Apr 29, 2000·Trends in Plant Science·T EulgemI E Somssich
May 30, 2001·Trends in Plant Science·H Knight, M R Knight
Feb 14, 2002·Plant, Cell & Environment·L. Xiong, J.-K. Zhu
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 12, 2002·Annual Review of Plant Biology·Jian-Kang Zhu
Dec 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Haejeong MoonDae-Jin Yun
Jan 2, 2003·Annals of Botany·Olga BlokhinaKurt V Fagerstedt
Feb 27, 2003·Plant Molecular Biology·Jixin DongZhixiang Chen
Sep 1, 2004·Current Opinion in Plant Biology·Bekir Ulker, Imre E Somssich
Oct 20, 2006·Nature·Yuan-Yue ShenDa-Peng Zhang
Mar 16, 2007·Amino Acids·M D Groppa, M P Benavides
Jan 8, 2009·Plant Physiology·Kazuo NakashimaKazuko Yamaguchi-Shinozaki
Jan 20, 2009·Plant & Cell Physiology·Eun-Kyoung JangKwang-Yeol Yang
Aug 29, 2009·Plant, Cell & Environment·Gad MillerRon Mittler
Mar 23, 2010·Trends in Plant Science·Paul J RushtonQingxi J Shen
May 22, 2010·The Plant Journal : for Cell and Molecular Biology·Xiaozhi RenZhizhong Gong
Aug 3, 2010·Trends in Plant Science·Christian DubosLoïc Lepiniec
Sep 28, 2010·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh Gill, Narendra Tuteja
Jun 29, 2011·The Plant Journal : for Cell and Molecular Biology·Yu-Jun HaoShou-Yi Chen
Jul 9, 2011·Journal of Experimental Botany·Charu Lata, Manoj Prasad
Oct 4, 2011·Biochimica Et Biophysica Acta·Ligang ChenDiqiu Yu

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Citations

May 21, 2016·Frontiers in Plant Science·Hui SongXingjun Wang
Dec 21, 2016·Journal of Integrative Plant Biology·Jingjing JiangJianhua Zhang
Dec 19, 2018·International Journal of Molecular Sciences·Ling WangYouxiong Que
Jan 1, 2019·Molecular Plant Pathology·Sujon SarowarJyoti Shah
Jun 29, 2018·Frontiers in Plant Science·Yuling BaiC G van der Linden
Feb 26, 2019·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Zhe WangHeping Cao
Feb 25, 2019·Molecular Biology Reports·Marina L DíazAlicia Carrera
Nov 30, 2019·International Journal of Molecular Sciences·Xue WangJing Guo
Jul 14, 2017·Frontiers in Plant Science·Fusheng SunGuangyuan He
Feb 23, 2020·International Journal of Molecular Sciences·Xiaorui LiJinyin Lv
Mar 3, 2020·Journal of Zhejiang University. Science. B·Edward MwandoChengdao Li
Jun 17, 2021·Molecular Biotechnology·Olha LakhnekoBogdan Morgun
Jul 3, 2021·International Journal of Molecular Sciences·Ieva UrbanavičiūtėMario A Pagnotta

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Methods Mentioned

BETA
transgenic
PCR
one-hybrid
electrophoresis
MDA
fluorescence microscopy

Software Mentioned

Excel
BLASTp
NetPhos
DNAMAN
CFX Manager
Mega

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