Expression of tetraspan protein CD63 activates protein-tyrosine kinase (PTK) and enhances the PTK-induced inhibition of ROMK channels.

The Journal of Biological Chemistry
Dao-Hong LinWen-Hui Wang

Abstract

In the present study, we tested the role of CD63 in regulating ROMK1 channels by protein-tyrosine kinase (PTK). Immunocytochemical staining shows that CD63 and receptor-linked tyrosine phosphatase alpha (RPTPalpha) are expressed in the cortical collecting duct and outer medulla collecting duct. Immunoprecipitation of tissue lysates from renal cortex and outer medulla or 293T cells transfected with CD63 reveals that CD63 was associated with RPTPalpha both in situ and in transfected cells. Expression of CD63 in 293T cells stimulated the phosphorylation of tyrosine residue 416 of c-Src but decreased the phosphorylation of tyrosine residue 527, indicating that expression of CD63 stimulates the activity of c-Src. Furthermore, c-Src was coimmunoprecipitated with RPTPalpha and CD63 both in 293T cells transfected with CD63 and in lysates prepared from native rat kidney. Potassium restriction had no effect on the expression of RPTPalpha, but it increased the association between c-Src and RPTPalpha in the renal cortex and outer medulla. We also used two-electrode voltage clamp to study the effect of CD63 on ROMK channels in Xenopus oocytes. Expression of CD63 had no significant effect on potassium currents in oocytes injected with ROMK1;...Continue Reading

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Citations

Aug 28, 2009·The Journal of Biological Chemistry·Dao-Hong LinWen-Hui Wang
Sep 24, 2010·Molecular Biology of the Cell·Monica CarmosinoMaria Svelto
Mar 3, 2009·Cardiovascular Research·Feng ZhangXin A Zhang
Jul 6, 2010·Current Opinion in Nephrology and Hypertension·Wen-Hui WangDao-Hong Lin
Dec 17, 2008·Molecular and Cellular Biology·Jenny SchröderPaul Saftig
Jun 1, 2011·PloS One·Michael Z TrikićLynda J Partridge
May 14, 2011·Journal of the American Society of Nephrology : JASN·Dao-Hong LinWen-Hui Wang
Oct 18, 2013·International Journal of Molecular Sciences·Bharat Bhusan PatnaikYeon Soo Han
Dec 4, 2010·Biochimica Et Biophysica Acta·Diego F FiolDietmar Kültz
Feb 15, 2011·Virology·Guangyu LiMonique R Ferguson
Nov 8, 2013·American Journal of Physiology. Renal Physiology·Dao-Hong LinWen-Hui Wang
Feb 9, 2021·Fish & Shellfish Immunology·Sumi JungJehee Lee

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