Expression of TGFalpha autocrine activity in human colon carcinoma CBS cells is autoregulated and independent of exogenous epidermal growth factor

Journal of Cellular Physiology
D JiangM G Brattain

Abstract

Autocrine transforming growth factor alpha (TGFalpha) activity and control mechanisms for its expression were examined in a representative clonal isolate (CBS4) of a well-differentiated human colon carcinoma cell line designated CBS. CBS4 cells expressed TGFalpha and its receptor, epidermal growth factor receptor (EGFr). Blockade of EGFr and TGFalpha by neutralizing antibodies inhibited clonal growth and the initiation of DNA synthesis from quiescence in CBS4 cells. Therefore, TGFalpha is an autocrine growth factor for CBS4 cells. Several studies have indicated that activation of the EGFr by exogenous EGF stimulates TGFalpha expression. However, in CBS4 cells EGF did not induce TGFalpha mRNA expression, indicating that EGF does not affect TGFalpha transcription in these cells. Exogenous treatment of exponentially growing cells with either EGF or EGFr blocking antibody enhanced release of TGFalpha protein into the conditioned medium. This indicated that the release of TGFalpha into the conditioned medium by exogenous EGF was at least partially due to the displacement of TGFalpha from the TGFalpha/EGFr complexes. Similarly to exponentially growing cells, the EGFr blocking antibody and EGF also enhanced TGFalpha release into the m...Continue Reading

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Citations

May 6, 2014·Biochimica Et Biophysica Acta·Maria-Ioanna EllinaNikos K Karamanos
Aug 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Göknil Pelin CoşkunŞ Güniz Küçükgüzel
Jun 22, 2006·Annals of Surgical Oncology·Krishdeep S ChadhaMilind M Javle
Mar 16, 2004·The Journal of Biological Chemistry·Patricia S KhoQiang Yu
Jan 10, 2002·Biochemical and Biophysical Research Communications·A El-ObeidMonica Nistér

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