Expression of the Alcaligenes eutrophus phbA gene in Escherichia coli using a positive selection vector based on phage Lambda lysis genes

Journal of Biotechnology
S KalousekU Bläsi

Abstract

A new positive selection vector, pGS23, based on the Lambda lysis cassette has been designed for efficient expression of homologous and heterologous genes in Escherichia coli. The plasmid permits controlled expression of a gene of interest under transcriptional control of the lac promoter with translation initiation of coding sequences directed by the phage T7 gene 10 ribosome binding site. The application of the vector system was tested for high level expression of the heterologous phbA gene of Alcaligenes eutrophus in E. coli.

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Citations

Jan 20, 2015·Metabolic Engineering·Karthik Sekar, Keith E J Tyo
Oct 31, 2002·Critical Reviews in Biotechnology·Young-Jun ChoiByong H Lee
Jan 1, 1996·Critical Reviews in Biotechnology·C P Kubicek
Mar 7, 2002·Journal of Advanced Nursing·Mary Gillespie
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Nov 29, 2007·Journal for Nurses in Staff Development : JNSD : Official Journal of the National Nursing Staff Development Organization·Pam Nicol, Melisa Young
Jul 28, 2007·Journal of Clinical Nursing·Li-Ling Hsu

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