Expression of the antiviral protein MxA in cells transiently perturbs endocytosis

Biochemical and Biophysical Research Communications
Shashidhar S Jatiani, Rohit Mittal

Abstract

MxA is an interferon-induced antiviral protein. Viral replication relies on the trafficking machinery of the host cell. Overexpression of MxA was found to perturb trafficking of internalized transferrin resulting in its accumulation in cells. Interestingly, this perturbation of endocytic trafficking was transient--with a maximal effect being seen 5-6 h after transfection. By 12 h after transfection the perturbation of endocytosis was seen to have subsided although MxA protein levels remained elevated even 24 h after transfection. The accumulation of transferrin is due to a block in transferrin recycling. It is further shown that MxA can physically associate with the endocytic protein dynamin, possibly accounting for the observed effect of MxA expression on transferrin endocytosis. These results uncover a hitherto unknown aspect of MxA action on trafficking processes within cells.

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Citations

Mar 20, 2009·The Journal of Biological Chemistry·J Frederic MushinskiJane B Trepel
Sep 19, 2006·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Silke StertzGeorg Kochs
Jun 30, 2010·American Journal of Reproductive Immunology : AJRI·Karen Racicot, Troy Ott
May 12, 2012·American Journal of Reproductive Immunology : AJRI·Karen RacicotTroy Ott
Jun 17, 2014·Traffic·Florian HoffRalf Jacob
Jun 12, 2013·Developmental and Comparative Immunology·Kate E GoossensAndrew G D Bean
Nov 14, 2006·Veterinary Pathology·B F PorterR W Storts

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