Expression of the arginine regulon of Escherichia coli W: evidence for a second regulatory gene.

Journal of Bacteriology
E C TheilE E Jones

Abstract

Effect of the M (modifier) gene of Escherichia coli W on the expression of wild-type structural genes of four arginine biosynthetic enzymes was studied by examining enzyme activity in cell-free extracts of cultures grown in minimal medium and medium containing arginine. The mutant M gene was originally identified as causing arginine-induced synthesis of acetylornithine delta-transaminase in a strain deficient for the enzyme. The strains used in this study received the mutant M gene by recombination. Noncoordinate repression has been demonstrated for two more enzymes of the arginine regulon of E. coli W and the M(-) gene increases the degree of noncoordinate repression for the regulon. Mutation of the M gene results in altered regulation of acetylornithine delta-transaminase, ornithine transcarbamylase, and acetylornithinase. In addition, a decreased growth rate is observed. It is proposed that the M gene is a regulatory gene. A model is presented to explain the data which involves changes in operator-repressor affinity for the structural genes and possibly for the gene controlling arginyl transfer ribonucleic acid synthetase.

References

Jun 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·H J Vogel, D F Bacon

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Citations

Jul 7, 1977·Molecular & General Genetics : MGG·D HaasT Leisinger
Feb 1, 1975·Japanese Journal of Microbiology·H MatsumotoT Tazaki
May 21, 1971·Biochemical and Biophysical Research Communications·F W WeyterE E Jones
Oct 1, 1978·Analytical Biochemistry·G F MiozzariR Hütter
Apr 2, 1974·Archives of Biochemistry and Biophysics·J T Billheimer, E E Jones

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