PMID: 9642194Jun 27, 1998Paper

Expression of the bglH gene of Lactobacillus plantarum is controlled by carbon catabolite repression

Journal of Bacteriology
R MarascoMargherita Sacco

Abstract

A newly identified bglH gene coding for a phospho-beta-glucosidase of Lactobacillus plantarum was isolated and expressed in Escherichia coli. The sequence analysis of the cloned DNA fragment showed an open reading frame encoding a 480-amino-acid protein with a calculated molecular mass of 53 kDa. The bglH gene was shown to be expressed on a monocistronic transcriptional unit. Its transcription was repressed 10-fold in L. plantarum cells grown on glucose compared to the beta-glucoside salicin as a sole carbon source. A catabolite-responsive element (CRE) spanning from -3 to +11 with respect to the transcriptional start point was found, and its functionality was assessed by mutational analysis. In vitro and in vivo DNA binding experiments suggested the occurrence of a DNA-protein complex at the CRE site, which would mediate glucose repression of bglH expression.

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Citations

Apr 6, 2002·FEMS Microbiology Letters·Rosangela MarascoMargherita Sacco
Apr 1, 2009·Microbiology·Jae-Han KimDavid A Mills
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Dec 18, 2013·FEMS Microbiology Letters·Herbert Michlmayr, Wolfgang Kneifel
Jun 27, 2001·Applied and Environmental Microbiology·L MuscarielloM Sacco
May 14, 1999·Current Opinion in Microbiology·J Stülke, W Hillen
Jul 7, 2011·Journal of Biotechnology·Mohamed Ali Abdel-RahmanKenji Sonomoto

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