Expression of the bumetanide-sensitive Na-K-Cl cotransporter BSC2 is differentially regulated by fluid mechanical and inflammatory cytokine stimuli in vascular endothelium

The Journal of Clinical Investigation
J N TopperM A Gimbrone

Abstract

In vascular endothelium, the electroneutral Na-K-Cl cotransport system is thought to function in the maintenance of a selective permeability barrier in certain vascular beds (e.g., brain), as well as in the preservation of endothelial homeostasis in the face of fluctuating osmotic conditions that may accompany certain pathophysiological conditions (e.g., diabetes mellitus). Here we demonstrate that the gene encoding the bumetanide-sensitive cotransporter BSC2, one of the two major isoforms of Na-K-Cl cotransporters present in mammalian cells, can be differentially regulated by inflammatory cytokines and fluid mechanical forces in cultured endothelium. Interleukin-1beta and tumor necrosis factor-alpha significantly upregulate expression of BSC2 mRNA and protein in human umbilical vein endothelial cells, a response that is inhibited by pretreatment with interferon-gamma. Steady laminar fluid shear stress, at a physiologic magnitude (10 dyn/cm2), is also able to induce and maintain elevated expression of BSC2 in cultured human umbilical vein endothelial cells, while a comparable time-averaged magnitude of turbulent fluid shear stress is not. In vivo, BSC2 mRNA is upregulated after intraperitoneal administration of bacterial endoto...Continue Reading

References

Aug 15, 1991·European Journal of Biochemistry·B R Williams
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P F DaviesM A Gimbrone
Dec 1, 1982·The Review of Scientific Instruments·S R BussolariM A Gimbrone
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·M CellierP Gros
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·J A Payne, B Forbush
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·J C XuB Forbush
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·N ResnickM A Gimbron
Feb 1, 1993·Circulation Research·P F Davies, S C Tripathi
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·N ResnickM A Gimbrone
Sep 1, 1995·Current Opinion in Nephrology and Hypertension·S C Hebert, S R Gullans

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Citations

Aug 20, 2010·Inflammatory Bowel Diseases·Mikihiro FujiyaEugene B Chang
May 7, 2003·Progress in Biophysics and Molecular Biology·Nitzan ResnickEfrat Wofovitz
Jul 9, 2002·Brain Research. Developmental Brain Research·Sumiko MikawaKohji Sato
Nov 5, 2002·Brain Research. Developmental Brain Research·Cong WangKohji Sato
Jul 10, 2007·Nature Medicine·Michael A Matthay, Xiao Su
Aug 16, 2011·Epilepsia·Edward H MaaKevin J Staley
Aug 15, 2000·Annual Review of Nutrition·J B German, R L Walzem
Mar 11, 2011·Journal of Vascular Research·Eirini Kefaloyianni, William A Coetzee
Nov 6, 2008·World Journal of Gastroenterology : WJG·Yutao Yan, Didier Merlin
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·J N TopperD Falb
Mar 6, 2007·Experimental and Molecular Pathology·William H HoffmanHorea Rus
Mar 19, 2009·European Journal of Clinical Investigation·N F Ramia, S I Kreydiyyeh
Aug 9, 2008·Microvascular Research·Luciano A Guerreiro-LucasChristopher A Mitchell
May 23, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·O Traub, B C Berk
Jan 11, 2007·Journal of Cellular Biochemistry·Su Jin KimJoo-Heon Yoon
Mar 7, 2019·Movement Disorders : Official Journal of the Movement Disorder Society·Yi-Ting HsuYijuang Chern
Oct 18, 2012·Physiological Reviews·Kenneth B Gagnon, Eric Delpire
Nov 6, 2018·Cerebral Cortex·Amandine FernandezYehezkel Ben-Ari
Oct 26, 2007·American Journal of Physiology. Cell Physiology·Elaine ChangAbdul I Barakat
Jun 3, 2015·Critical Care Medicine·Hsueh-Ju LinKun-Lun Huang
Apr 3, 1999·The Journal of Biological Chemistry·K HikiJ R Gamble
Dec 21, 2000·American Journal of Physiology. Cell Physiology·J SuvatneM E O'Donnell
Mar 10, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·C M LiedtkeT S Cole
Feb 26, 2016·American Journal of Physiology. Renal Physiology·Xiaohua HuangBranko Braam
Dec 10, 2002·Journal of Applied Physiology·Lynn M IwamotoRandal K Wada
Nov 18, 2000·American Journal of Physiology. Cell Physiology·G SuD Sun
May 6, 2020·International Journal of Molecular Sciences·Arturo NavasAntonio Rodríguez-Diéguez
Nov 14, 1998·The American Journal of Physiology·L M MaglovaJ M Russell
Dec 18, 2016·The Journal of Thoracic and Cardiovascular Surgery·Chou-Chin LanKun-Lun Huang
Dec 28, 2010·Journal of Proteome Research·Sandra Burghoff, Jürgen Schrader

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