Expression of the C-type lectins DC-SIGN or L-SIGN alters host cell susceptibility for the avian coronavirus, infectious bronchitis virus.

Veterinary Microbiology
Yueting ZhangGary R Whittaker

Abstract

Infectious bronchitis virus (IBV), an avian coronavirus, is a cause of great economic loss in the poultry industry. The virus mainly infects respiratory epithelium, but can be also detected in other organs. The functional receptor for the virus has not been found and field strains of IBV do not infect conventional cell lines. Recently, it has been shown that the C-type lectins DC-SIGN/L-SIGN can promote entry of several coronaviruses. Here we examine whether DC-SIGN/L-SIGN are entry determinants for IBV. We show that by introducing human DC-SIGN/L-SIGN into non-permissive cells, infection by the IBV is dramatically increased. DC-SIGN mediated infection was inhibited by mannan and anti-lectin antibodies, and was independent of sialic acid levels on the cell. Enhancement of IBV infection also occurred for different serotypes of IBV. Our findings demonstrated that even in the absence of avian-specific receptor, DC-SIGN-like lectins are capable of mediating efficient IBV infection.

References

Jun 7, 2002·Journal of Virology·Carmen P AlvarezRafael Delgado
Mar 14, 2003·Journal of Virology·Stefan PöhlmannJane A McKeating
Feb 14, 2004·Journal of Virology·Aymeric de ParsevalBenhur Lee
Jun 15, 2004·Nature Structural & Molecular Biology·Yuan GuoKurt Drickamer
Oct 22, 2004·Proceedings of the National Academy of Sciences of the United States of America·Scott A JeffersKathryn V Holmes
Apr 11, 2006·The Journal of General Virology·Christine WinterGeorg Herrler
Jul 11, 2007·Avian Pathology : Journal of the W.V.P.A·H Y ChenH C Chen
May 7, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Ui-Soon KhooC L Steve Lin
May 17, 2008·Avian Pathology : Journal of the W.V.P.A·G D Raj, R C Jones
Sep 19, 2008·Journal of Virology·Andrew D Regan, Gary R Whittaker
Jun 24, 2009·Experimental Biology and Medicine·Patrick C Y WooKwok-Yung Yuen

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Citations

Dec 3, 2014·Virus Research·I N Ambepitiya WickramasingheM H Verheije
Jul 21, 2012·Viruses·Sandrine BelouzardGary R Whittaker
Sep 5, 2014·Journal of Virology·Aleksandra MilewskaKrzysztof Pyrc
Jul 24, 2020·International Journal of Molecular Sciences·Dimitri Leonid Lindenwald, Bernd Lepenies
Oct 30, 2020·FEMS Microbiology Reviews·Jean K MilletGary R Whittaker
May 7, 2021·FEMS Microbiology Reviews·Jean K MilletGary R Whittaker

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Methods Mentioned

BETA
transfection
PCR

Software Mentioned

BLAST

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