Expression of the Caldariomyces fumago chloroperoxidase in Aspergillus niger and characterization of the recombinant enzyme

The Journal of Biological Chemistry
A ConesaPeter J Punt


The Caldariomyces fumago chloroperoxidase was successfully expressed in Aspergillus niger. The recombinant enzyme was produced in the culture medium as an active protein and could be purified by a three-step purification procedure. The catalytic behavior of recombinant chloroperoxidase (rCPO) was studied and compared with that of native CPO. The specific chlorination activity (47 units/nmol) of rCPO and its pH optimum (pH 2.75) were very similar to those of native CPO. rCPO catalyzes the oxidation of various substrates in comparable yields and selectivities to native CPO. Indole was oxidized to 2-oxindole with 99% selectivity and thioanisole to the corresponding R-sulfoxide (enantiomeric excess >98%). Incorporation of (18)O from labeled H(2)18O(2) into the oxidized products was 100% in both cases.


Nov 1, 1979·The Biochemical Journal·M D Corbett, B R Chipko
Aug 1, 1992·Trends in Biotechnology·J S Dordick
Jan 1, 1992·Methods in Enzymology·Peter J Punt, Cees van den Hondel
Aug 30, 1991·Biochemical and Biophysical Research Communications·A K Black, C A Reddy
May 1, 1987·Archives of Biochemistry and Biophysics·P KenigsbergLowell P Hager
Jan 1, 1987·Molecular & General Genetics : MGG·W van HartingsveldtCees van den Hondel
Aug 11, 1987·Biochemistry·S KobayashiA P Schaap
Sep 26, 1995·Biochemistry·Q ZongLowell P Hager
Jun 15, 1995·FEMS Microbiology Letters·W BurdK-H van Pée
Jul 1, 1995·Bioscience, Biotechnology, and Biochemistry·H Sawai-HatanakaY Shibano
Jun 1, 1995·Archives of Biochemistry and Biophysics·V P MillerP R Ortiz de Montellano
Feb 13, 1993·Biochimica Et Biophysica Acta·J W van SchijndelR Wever
Feb 23, 1996·The Journal of Biological Chemistry·J N Rodriguez-LopezR N Thorneley
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·D W BrownT J Leonard
Jun 21, 1996·The Journal of Biological Chemistry·S L Newmyer, P R de Montellano
Sep 6, 1996·The Journal of Biological Chemistry·R D LibbyA K Phipps
Oct 14, 1996·Biochemical and Biophysical Research Communications·M TanakaI Morishima
Mar 1, 1996·Applied and Environmental Microbiology·P StewartM Tien
May 1, 1997·The American Journal of Occupational Therapy : Official Publication of the American Occupational Therapy Association·B CoxR A Mallory
Jul 17, 1998·Current Opinion in Chemical Biology·A T Smith, N C Veitch
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·X YiLowell P Hager
Jul 6, 2000·Applied and Environmental Microbiology·A ConesaPeter J Punt
May 31, 1996·The Journal of Organic Chemistry·Frederick J. Lakner, Lowell P Hager
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN


Feb 28, 2009·Archives of Microbiology·Kateryna ZelenaRalf Günter Berger
Apr 22, 2006·Applied Microbiology and Biotechnology·Martin Hofrichter, René Ullrich
May 13, 2009·Applied Microbiology and Biotechnology·Marek J PecynaMartin Hofrichter
Apr 12, 2002·Trends in Biotechnology·Peter J PuntCees van den Hondel
Dec 12, 2001·Journal of Biotechnology·Ana ConesaCees A M J J van den Hondel
Nov 26, 2002·FEMS Microbiology Letters·Nurit Bar-NunAlfred M Mayer
Jun 1, 2002·Current Opinion in Chemical Biology·Patrick C Cirino, Frances H Arnold
Aug 6, 2004·Applied and Environmental Microbiology·René UllrichMartin Hofrichter
Jul 16, 2005·Applied Microbiology and Biotechnology·Xavier O WeeninkArthur F J Ram
Feb 11, 2003·The Journal of Biological Chemistry·Xianwen YiLowell P Hager
Apr 1, 2014·Applied and Environmental Microbiology·Patricia Molina-EspejaMiguel Alcalde
May 15, 2008·Applied Biochemistry and Biotechnology·Abhijeet P Borole, Brian H Davison
Nov 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alexander Veljko FejzagićThomas Classen

Related Concepts

Aspergillus niger
Chloride Peroxidase
Yeast Proteins
Proteins, Recombinant DNA
Substrate Specificity

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.