Expression of the cholinergic signal-transduction pathway components during embryonic rat heart development

The Anatomical Record
D FrancoW H Lamers


Previous studies showed that acetylcholinesterase (AChE) activity is present in the downstream (arterial) part of the embryonic chick and rat heart, but its functional significance was unclear. To establish whether other components of a cholinergic signal-transduction pathway are present in the embryonic heart, we localised the mRNAs encoding choline acetyltransferase (ChAT), acetylcholinesterase (AChE), and the muscarinic receptor isoforms (mAChRs; m1-m5). Messenger RNA detection and localisation by in situ hybridisation and reverse transcriptase-polymerase chain reaction were employed. Expression of ChAT and AChE mRNAs was observed from 15 embryonic days onward in the neural tissue covering the dorsocranial wall of the atria. Muscarinic receptors (m1, m2, m4) were observed at the same localisation as AChE and ChAT mRNAs, both during embryogenesis and after birth. In addition, m1 and m4 mAChRs showed a low level of expression in the atrial myocardium during the fetal period. No expression of the m3 or the m5 mAChRs was observed in or near the embryonic hearts. ChAT, AChE, and mAChRs (m1, m2, m4) mRNAs always colocalised in the cardiac ganglia. However, none of these mRNAs was found at a detectable level in the outflow tract an...Continue Reading


Feb 3, 2011·Cardiovascular Research·Ryota TeradaHideko Kasahara
May 19, 2004·British Journal of Pharmacology·Zhiguo WangHuizhen Wang
Apr 18, 1998·Circulation Research·A F MoormanW H Lamers
Dec 14, 2011·PloS One·Daniela L BuscariolloS A Rivkees
Nov 19, 2004·Cell and Tissue Research·Magdalena DvorakovaWolfgang Kummer
Jul 13, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·G A Porter, S A Rivkees


Sep 18, 1992·Cell·W J Tang, A G Gilman
Aug 1, 1992·Circulation Research·F de JongA F Moorman
Jan 1, 1990·Annual Review of Pharmacology and Toxicology·E C HulmeN J Buckley
Nov 1, 1990·The Anatomical Record·W H LamersA F Moorman
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H SoreqY Lipidot-Lifson
Jan 1, 1990·Anatomy and Embryology·U Drews, W Mengis
Jan 1, 1989·Life Sciences·L MeiH I Yamamura
Jul 1, 1989·Circulation Research·C Nyquist-Battie, K Trans-Saltzmann
Apr 1, 1987·Journal of the Autonomic Nervous System·J C HancockM W Hougland
May 1, 1986·Journal of Molecular and Cellular Cardiology·C ArgüelloB Valenzuela
Mar 1, 1970·The American Journal of Cardiology·E CoraboeufG Le Douarin
May 1, 1980·Circulation Research·W J MarvinR Roskoski
Jan 1, 1980·Neuroscience·I W ChubbG H White
Jan 1, 1982·Annual Review of Neuroscience·J Massoulié, S Bon
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·H SoreqH Zakut
Jan 1, 1993·Brain Research. Molecular Brain Research·J C LauterbornC M Gall
Nov 1, 1994·Trends in Cardiovascular Medicine·A F Moorman, W H Lamers

Related Concepts

Establishment and Maintenance of Localization
Embryonic Heart
Biochemical Pathway
Entire Cardiac Ganglia
CYP2C19*6 Allele
Signal Transduction Pathways

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.