PMID: 7520026Aug 15, 1994Paper

Expression of the co-stimulatory molecule B7 on melanoma cells

International Journal of Cancer. Journal International Du Cancer
P HerseyW D Thomas

Abstract

The induction of T-cell responses against tumor cells is believed to depend on both recognition of antigen and receipt of co-stimulatory signals from interaction of ligands such as B7 with its receptors CD28 or CTLA-4 on T cells. In the present study the expression of B7 on cultured human melanoma cells was studied at the mRNA level by reverse PCR analysis and surface expression by flow cytometric analysis with monoclonal antibodies (MAbs). PCR analysis revealed mRNA for B7 in 3 of 6 (50%) cultured primary melanoma and 8 of 19 (42%) cultures of metastatic melanoma. Analysis of B7 expression by flow cytometry using the BB1 MAb revealed low levels of expression in 3 of 10 melanoma that had mRNA for B7. In 2 of the latter (but not 4 other PCR+ lines) expression could be increased by culture in GM-CSF, IL-2, IFN-gamma and IFN-alpha 2. Our results indicate that although mRNA for B7 is present in 40-50% of melanoma cell lines, expression at the protein level is at low or undetectable levels in the majority of the cell lines. Expression of B7 protein was also not detected in studies on tissue sections from 11 primary and 9 metastatic melanomas.

References

May 8, 1992·International Journal of Cancer. Journal International Du Cancer·Q Chen, P Hersey
Sep 1, 1991·The Journal of Experimental Medicine·P S LinsleyJ A Ledbetter
Jun 1, 1991·Cancer Metastasis Reviews·C D Platsoucas
Jun 1, 1990·Immunology Today·C H JuneC B Thompson
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P S LinsleyJ A Ledbetter
Apr 15, 1988·International Journal of Cancer. Journal International Du Cancer·E B BröckerC Sorg
Mar 15, 1988·International Journal of Cancer. Journal International Du Cancer·P HerseyG D'Alessandro
May 1, 1981·Molecular Immunology·S CarrelR S Accolla
Dec 1, 1983·American Journal of Clinical Pathology·K F BradstockW G Hughes
Mar 1, 1993·The Journal of Experimental Medicine·M AzumaL L Lanier
Apr 1, 1993·The Journal of Experimental Medicine·E A Ranheim, T J Kipps
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S BaskarL H Glimcher
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C D GimmiL M Nadler

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Citations

Dec 11, 1996·International Journal of Cancer. Journal International Du Cancer·W D ThomasP Hersey
Jan 8, 1998·International Journal of Cancer. Journal International Du Cancer·S B HoschK Pantel
Nov 1, 1996·Seminars in Surgical Oncology·C L Slingluff
Jul 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T TatsumiN Hayashi
Mar 19, 1999·Immunology Today·J O'ConnellF Shanahan
Jun 6, 2000·Advanced Drug Delivery Reviews·Z Eshhar, C J Fitzer-Attas
Sep 12, 2001·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·J M WroblewskiJ R Yannelli
Dec 4, 2003·Biochimica Et Biophysica Acta·Simone MocellinFrancesco M Marincola
Mar 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·F VánkyE Klein
Jun 1, 1995·Immunological Reviews·K E HellströmL Chen
Jul 28, 1995·International Journal of Cancer. Journal International Du Cancer·R W DenfeldJ C Simon
Sep 4, 1995·International Journal of Cancer. Journal International Du Cancer·K J RadfordP Hersey
May 1, 2009·Human Immunology·Marta E PolakIan A Cree
Oct 19, 2016·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Marie de CharetteRoch Houot
Jul 9, 2016·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·Joerg U SchmohlHelga Schmetzer
Oct 1, 1995·Cancer Control : Journal of the Moffitt Cancer Center·C L Slingluff
Feb 21, 2006·Cancer Research·Iñigo TirapuIgnacio Melero
Apr 13, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Gregory LizéePatrick Hwu

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