PMID: 8468005Feb 1, 1993Paper

Expression of the Escherichia coli ftsZ gene: trials and tribulations of gene fusion studies

Genetical Research
A Robin, R D'Ari

Abstract

The ftsZ gene of Escherichia coli, which codes for an essential cell division protein, is subjected to multiple regulation, as shown in part with studies using an ftsZ::lacZ operon fusion located on phage lambda JFL100. Using this same fusion, we sought to isolate regulatory mutants overexpressing ftsZ by selecting mutants able to grow on lactose. One Lac+ mutant was obtained which overexpressed the ftsZ::lacZ fusion 70-fold. The mutation responsible for the overexpression lies in a new gene, cot, located near 56 min on the E. coli genetic map. The cot mutation probably affects the transcription of a chromosomal open reading frame, ORF1, lying downstream of the bioA gene and adjacent to the ftzZ::lacZ fusion of the lambda JFL100 prophage integrated at att lambda. Using an ftsZ84(Ts) strain, in which there was a double selection for overexpression of both ftsZ::lacZ and ftsZ+, no Lac+Tr mutants were obtained from 3.6 x 10(10) bacteria; the introduction of a mutL allele, increasing spontaneous base substitution mutation rates 75-fold, did not permit us to isolate such a mutant. We conclude that Lac+ ftsZ-constitutive mutations cannot be obtained in lambda JFL100 lysogens by a single base substitution.

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Citations

Dec 6, 2011·PLoS Neglected Tropical Diseases·Zhiru LiClotilde K Carlow
Jan 10, 2012·Environmental Microbiology·I F EscapaM A Prieto
Nov 13, 2012·PLoS Neglected Tropical Diseases·Jessica N RicaldiMichael A Matthias
Nov 29, 1995·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J R Guest
Jul 20, 2016·Royal Society Open Science·Folgert Karsdorp, Antal van den Bosch
Jul 22, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D ThieffryJ Collado-Vides

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