PMID: 9179927May 1, 1997Paper

Expression of the facilitated glucose transporters (GLUT1 and GLUT3) by a choriocarcinoma cell line (JAr) and cytotrophoblast cells in culture

Placenta
L H ClarsonC P Sibley

Abstract

The expression of GLUT1 and GLUT3 mRNA and protein in human placental trophoblast-derived cells was investigated. A dividing choriocarcinoma derived cell line (JAr) was compared to differentiating cytotrophoblast cells, isolated from human term placenta, following 18 (mononucleate) and 66 h (multinucleate) in culture. JAr cells treated with 8-bromoadenosine, which inhibits growth and induces differentiation, were also studied. GLUT1 mRNA and protein expression were similar in the four groups of cells. However, GLUT3 mRNA expression was significantly higher (six- to sevenfold) in both control and 8-bromoadenosine-treated JAr cells compared to cytotrophoblast cells and was also significantly higher in untreated versus treated JAr cells. Western blotting showed that GLUT3 protein was undetectable in either cytotrophoblast cell groups, but was abundant in both groups of JAr cells. GLUT3 protein in JAr cells treated with 8-bromoadenosine was also significantly lower than in untreated JAr cells, in agreement with the mRNA data. We conclude that GLUT1 expression is unaffected by either growth or differentiation of trophoblast cells whereas GLUT3 expression is associated with dividing cells. We propose that in the placenta, GLUT3 may b...Continue Reading

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Citations

Jun 21, 2005·Histochemistry and Cell Biology·Emin Turkay KorgunRamazan Demir
Jun 6, 2000·Advanced Drug Delivery Reviews·G T KnippM J Soares
Mar 1, 2000·Clinical Obstetrics and Gynecology·N P Illsley
Mar 29, 2000·Journal of Bacteriology·S HeilandH Lichtenberg
Aug 13, 2015·Placenta·Carolyn J P JonesJohn D Aplin
Aug 15, 2009·Journal of Cellular Physiology·Sara Rodríguez-EnríquezRafael Moreno-Sánchez
Apr 7, 1998·Biochimica Et Biophysica Acta·P D PrasadV Ganapathy
Feb 29, 2000·Placenta·N P Illsley
Oct 28, 2004·Thrombosis Research·Neil M GudeRoger G King
Sep 16, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·T HahnG Desoye
Dec 30, 2018·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Nicholas P Illsley, Marc U Baumann

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