Expression of the gap gene encoding glyceraldehyde-3-phosphate dehydrogenase of Streptomyces aureofaciens requires GapR, a member of the AraC/XylS family of transcriptional activators

Microbiology
O SprusanskýJan Kormanec

Abstract

Expression of the gap gene encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is developmentally regulated, and induced by glucose in Streptomyces aureofaciens. A gene, gapR, encoding a protein similar to the AraC/XylS family of bacterial transcriptional regulators was identified upstream of gap. The gapR gene was constitutively expressed from a single promoter during the course of differentiation. By integrative transformation, via double crossover, a stable null mutant of the gapR gene was obtained. The mutation only slightly affected growth, and had no effect on differentiation of S. aureofaciens. However, transcription of the GAPDH-encoding gap gene was substantially reduced in the S. aureofaciens DeltagapR null mutant, irrespective of carbon source used. Though GAPDH activity was about 1.5-fold lower in the mutant, the substantial enzyme activity remained, suggesting the presence of a second GAPDH which is sufficient to ensure growth. The GapR protein, overproduced in Escherichia coli, was shown to bind upstream of the gap-P promoter region. The results indicate a direct role of GapR in regulation of gap expression in S. aureofaciens.

References

Apr 1, 1992·Gene·F Wright, M J Bibb
Sep 11, 1987·Nucleic Acids Research·H A Baylis, M J Bibb
Jan 1, 1982·Methods in Enzymology·L D Byers
Aug 1, 1997·Journal of Bacteriology·F D SetaG Branlant
Jun 1, 1998·Microbiology·Keith F Chater

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Citations

Jun 21, 2015·Biochimica Et Biophysica Acta·Alba Romero-RodríguezSergio Sánchez
Jun 22, 2018·Journal of Applied Microbiology·M GoswamiJ Jass
Aug 28, 2007·Journal of Bacteriology·Steffen SchlagFriedrich Götz
Jun 3, 2021·International Journal of Molecular Sciences·Karolina KoteckaAneta Agnieszka Bartosik

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