Expression of the glial cell line-derived neurotrophic factor gene in rat brain after transient MCA occlusion

Brain Research
K Abe, T Hayashi

Abstract

Change of the glial cell line-derived neurotrophic factor (GDNF) gene expression in rat brain was examined after transient middle cerebral artery (MCA) occlusion of adult rats. Northern blot analysis showed that the mRNA began to be induced in the occluded MCA from 1 h of reperfusion with a peak at 3 h, and almost diminished by 1 day of reperfusion. Immunohistochemical analysis with brain sections showed an expression of GDNF-like immunoreactivity in neurons of the cerebral cortex and caudate after 90 min of ischemia in a similar way to the mRNA, but the staining was more disseminated and stronger in the cerebral cortex than the caudate. No glial cell was stained in the brain sections. The present results indicate that the GDNF gene was expressed in an early stage of reperfusion in neuronal cells of the MCA territory, but that the staining property was different between in the cerebral cortex and caudate.

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Citations

Jan 29, 2013·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Zhongmei ChenGuoguang Peng
Jun 24, 2004·Neurobiology of Aging·Woei-Cherng ShyuHung Li
Dec 18, 2002·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Tomoaki IkedaBen H Choi
Mar 29, 2007·Taiwanese Journal of Obstetrics & Gynecology·Sheng-Hsien ChenFong-Ming Chang
Dec 22, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Y H ChiangY Wang
Dec 22, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·H KitagawaK Abe
Jun 22, 2002·Annals of the New York Academy of Sciences·Y WangJ Hoffer
Jul 31, 2001·Experimental Neurology·A SarabiM Morales
Jun 14, 2005·Brain Research. Molecular Brain Research·Johann SellnerUta Meyding-Lamadé
Jun 8, 2012·Growth Factors·Emília P DuarteCarlos B Duarte
Dec 9, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kazuhiko KurozumiHirofumi Hamada
Oct 24, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·K Abe
Mar 26, 2005·Cell Transplantation·Yung-Hsiao ChiangYun Wang
Mar 7, 2001·Nature Medicine·M TamataniM Tohyama
Apr 5, 2003·Stroke; a Journal of Cerebral Circulation·Ulkan KilicMathias Bähr
Sep 8, 2001·Stroke; a Journal of Cerebral Circulation·Y WangB J Hoffer

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