PMID: 8997718Jan 1, 1997Paper

Expression of the heme biosynthetic pathway genes hemCD, hemH, hemM, and hemA of Escherichia coli

FEMS Microbiology Letters
P M McNicholasR P Gunsalus

Abstract

Little is known about the control of latter steps of heme biosynthesis in Escherichia coli. In this study we examined the transcriptional regulation of genes that encode two intermediate heme pathway enzymes, porphobilinogen deaminase (hemC) and uroporphyrinogen III cosynthase (hemD), and the final enzyme of the pathway, ferrochelatase (hemH). We also reexamined the regulation of hemA and the gene located immediately upstream of hemA, hemM. The regulatory regions of hemC, hemH, hemA and hemM were fused to lacZ. The resultant operon fusions were inserted into the E. coli chromosome in single copy and expression monitored under conditions of oxygen and heme limitation. Expression of hemM appeared constitutive under the conditions tested here. In contrast, expression of hemCD, hemH and hemA were shown to be mildly regulated in response to heme availability. Thus, transcription of four of the nine genes of the E. coli heme pathway appears to be only mildly regulated in response to heme limitation.

References

Jun 1, 1992·Trends in Biochemical Sciences·D JahnD Söll
Jun 5, 1991·Journal of Molecular Biology·K MiyamotoH Inokuchi
May 1, 1994·Journal of Bacteriology·W ChenS D Cosloy

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Citations

Jun 21, 2011·Applied Microbiology and Biotechnology·Angelique C W FrankenSandra de Weert
Mar 31, 2010·The Journal of Biological Chemistry·Andries van MourikMarc M S M Wösten
Feb 1, 2017·Microbial Cell Factories·Huan FangDawei Zhang

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