Expression of the human carbonic anhydrase I gene is activated late in fetal erythroid development and regulated by stage-specific trans-acting factors

British Journal of Haematology
H J BradyP H Butterworth

Abstract

Using flow cytometric analysis of red cells from different stages of ontogeny with anti-CAI antibody, it was shown that the human carbonic anhydrase I (HCAI) gene product appears in a developmental stage-specific manner. Virtually no CAI protein was detectable in fetal red cells prior to birth. However, at about the time of normal delivery (40 weeks gestation) CAI production is switched on. The proportion of cells containing CAI reaches adult levels during the second half of the first year of life. Northern analysis suggests that the appearance of CAI protein results directly from the activation of the gene and the production of new mRNA. A transient heterokaryon system was set up by fusing the erythroleukaemic cell lines MEL C88 (a mouse cell line in which CAI is expressed) and K562 SAI (a human cell line with an embryonic/fetal phenotype, not expressing CAI). SP6 RNAase mapping of RNA from the fused cells showed activation of the human CAI gene. This shows the developmental stage-specific expression of HCAI to be regulated by trans-acting factors.

References

Apr 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S S SpicerR E Tashian
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 1, 1989·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R E Tashian
Aug 1, 1989·Molecular and Cellular Biology·P FraserP Curtis
Mar 11, 1987·Nucleic Acids Research·J H BarlowP H Butterworth
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T EvansG Felsenfeld
Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·C FriendT Sato
Jun 1, 1980·European Journal of Biochemistry·C Auffray, F Rougeon
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·E J BenzR Hoffman

❮ Previous
Next ❯

Citations

Apr 1, 1996·Clinical Biochemistry·S Aliakbar, P R Brown
Jul 21, 1998·Thyroid : Official Journal of the American Thyroid Association·H HoriS Ito
Mar 18, 2009·Journal of Perinatal Medicine·Christoph ScholzFranz Kainer
Jun 29, 2012·Transfusion·Eugenia Prus, Eitan Fibach
Jun 1, 1993·Differentiation; Research in Biological Diversity·J SowdenY Edwards
Feb 13, 2018·Human Molecular Genetics·Samuel LessardGuillaume Lettre
Mar 1, 1996·European Journal of Biochemistry·F DrummondY H Edwards
Aug 5, 2017·Journal of Translational Medicine·Jaira F de VasconcellosJeffery L Miller
May 1, 2002·Molecular Endocrinology·Ji-Won KimHaeyoung Suh-Kim
Jun 28, 2017·Proceedings of the National Academy of Sciences of the United States of America·Jaira F de VasconcellosJeffery L Miller
Sep 20, 1996·Journal of Chromatography. B, Biomedical Applications·N Bergenhem

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved