PMID: 6159642Sep 1, 1980Paper

Expression of the human fibroblast interferon gene in Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America
T TaniguchiM Ptashne

Abstract

We applied the method of Guarente et al. [Guarente, L., Lauer, G., Roberts, T.M. & Ptashne, M. (1980) Cell 20, 543-553] to construct plasmids that direct expression in Escherichia coli of the human fibroblast interferon (F-IF) gene. Two plasmids were recovered. One directs efficient synthesis of a protein whose primary sequence is that of pre-F-IF and the other, that of mature F-IF. Extracts of bacteria synthesizing mature F-IF display antiviral activity characteristic of human F-IF. This activity is lower than that expected from the differential rate of synthesis of the protein. We have detected no such activity in extracts of bacteria synthesizing pre-F-IF.

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T M RobertsM Ptashne
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T M RobertsM Ptashne
Feb 11, 1976·Proceedings of the National Academy of Sciences of the United States of America·E Knight
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Citations

Aug 6, 2008·Applied Biochemistry and Biotechnology·Dasari Venkata Krishna RaoAdibhatla Kali Satya Bhujanga Rao
Jan 1, 1983·The International Journal of Biochemistry·I Suominen, P Mäntsälä
Oct 13, 1983·Biochimica Et Biophysica Acta·K SiggensD Burke
Jun 24, 1985·Biochimica Et Biophysica Acta·G GrossH Blöcker
May 1, 1983·Gene·I PalvaC Weissmann
Nov 3, 2001·International Journal of Antimicrobial Agents·E De Clercq
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S Ohno, T Taniguchi
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G JayE Jay
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P SlocombeD C Burke
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J S EmtageP A Lowe
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L D SimonG Binkowski
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S H Shen
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D F MarkL S Lin
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E JayM Dawood
Jan 1, 1984·Biotechnology & Genetic Engineering Reviews·M D Edge, R Camble
Apr 1, 1982·The Journal of Experimental Medicine·D L SlateF H Ruddle
Jan 1, 1982·Journal of Interferon Research·E Knight, D Fahey
Jan 1, 1982·Journal of Interferon Research·A R NeurathP M Pitha
Jan 1, 1983·Journal of Interferon Research·K G McCullaghM Houghton
Oct 1, 1987·Journal of Interferon Research·T Taniguchi
Aug 1, 1988·Journal of Interferon Research·P J ZavodnyP J Leibowitz
May 11, 1983·Nucleic Acids Research·S I FeinsteinY Mory
Jan 25, 1985·Nucleic Acids Research·B SchaffhausenT M Roberts

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