Expression of the human mu opioid receptor in a stable Sf9 cell line

Journal of Biotechnology
Juliette KempfD Massotte

Abstract

The cDNA that encodes the human mu opioid receptor (hMOR) has been cloned and expressed in Spodoptera frugiperda (Sf9) cells using a nonlytic vector system. The coding sequence fused to the cleavable glycoprotein signal peptide gp 64, and a C-terminal histidine tag was placed under the transcriptional control of the Orgyia pseudotsugata multicapsid nucleopolyhedrosis virus immediate-early 2 (OpIE2) promoter. Transfected cells were selected using Zeocin resistance and the receptor was constitutively expressed at approximately 12000 receptors per cell. Immunofluorescence images illustrated that more than 75% of the Sf9 cells expressed hMOR at the plasma membrane. This is the first report of the constitutive and heterologous expression of a G protein-coupled receptor in a stably transfected Sf9 cell line, under the control of the OpIE2 promoter.

References

Aug 1, 1995·Quarterly Reviews of Biophysics·R Grisshammer, C G Tate
Nov 14, 1997·Current Opinion in Biotechnology·L McCarroll, L A King
Mar 4, 2000·Peptides·A L VaccarinoA J Kastin
Jan 11, 2001·Peptides·A L Vaccarino, A J Kastin
Mar 14, 2001·Drug and Alcohol Dependence·L McCarthyT J Rogers

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Citations

Apr 30, 2003·Journal of Structural Biology·Patrick J Loll
Jan 31, 2012·Acta Pharmacologica Sinica·Qiang Zhao, Bei-li Wu
Jun 26, 2013·Microscopy·Yoshiyuki Arai, Takeharu Nagai
Dec 18, 2013·Journal of Biotechnology·Xiao ShenFlorian M Wurm
Aug 13, 2014·Proceedings of the National Academy of Sciences of the United States of America·Nicole E SeahArthur Landy
Jan 1, 2012·Scientifica·Shintaro Sasuga, Toshiya Osada
May 6, 2011·European Journal of Cell Biology·Ralf SchüleinWalter Rosenthal
Aug 14, 2010·Pharmacology & Therapeutics·Erich H Schneider, Roland Seifert
Jun 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Linda G ZachariassenAnders S Kristensen
Sep 12, 2006·Biochemical and Biophysical Research Communications·Arun Kumar ShuklaHartmut Michel
Apr 17, 2013·Biotechnology and Applied Biochemistry·Katja NäreojaJohnny Näsman
Jul 19, 2017·Proceedings of the National Academy of Sciences of the United States of America·Gustavo FuertesEdward A Lemke
Nov 29, 2020·Biotechnology Journal·Eduard Puente-MassaguerFrancesc Gòdia
Aug 23, 2020·Insect Biochemistry and Molecular Biology·Xien ChenSubba Reddy Palli
Feb 1, 2005·Biochimica Et Biophysica Acta·Aaron Kerman, Vettai S Ananthanarayanan

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