Expression of the insecticidal protein gene from Bacillus thuringiensis subsp. aizawai in Bacillus subtilis and in the thermophile Bacillus stearothermophilus by using the alpha-amylase promoter of the thermophile.

Applied and Environmental Microbiology
K Nakamura, T Imanaka

Abstract

Expression of the insecticidal protein gene from Bacillus thuringiensis subsp. aizawai IPL7 in B. subtilis MI113 and B. stearothermophilus SIC1 was examined. Production of the protein (130 kilodaltons [KDa]) was analyzed by its reaction with antibody against the insecticidal proteins of the parental B. thuringiensis. When the original gene containing its own promoter was subcloned in B. subtilis, only a small amount of the protein was produced. Therefore, both the promoter for the B. stearothermophilus alpha-amylase gene and the insecticidal protein gene were inserted in a repA (low-copy-number) plasmid to yield the recombinant plasmid pTBT-Pamy. B. subtilis MI113 carrying pTBT-Pamy produced more of the 130-kDa protein (about 10(4) molecules per cell) at 37 degrees C. In contrast, B. stearothermophilus SIC1 carrying pTBT-Pamy produced a small amount of 130-kDa protein (10(2) to 10(3) molecules per cell) at 55 degrees C.

References

Jun 1, 1989·Microbiological Reviews·H Höfte, H R Whiteley
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H LiaoR Hageman
Jan 1, 1986·Annual Review of Microbiology·H R Whiteley, H E Schnepf
Jul 1, 1985·Journal of Bacteriology·R NakajimaS Aiba
Oct 14, 1970·Journal of Molecular Biology·M Mandel, A Higa
Dec 1, 1988·Applied and Environmental Microbiology·M ZhangT Imanaka

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