Expression of the IP3R1 promoter-driven nls-lacZ transgene in Purkinje cell parasagittal arrays of developing mouse cerebellum

Journal of Neuroscience Research
D FurutamaTeiichi Furuichi

Abstract

The cerebellar Purkinje cell monolayer is organized into heterogeneous Purkinje cell compartments that have different molecular compositions. Here we describe a transgenic mouse line, 1NM13, that shows heterogeneous transgene expression in parasagittal Purkinje cell arrays. The transgene consists of a nuclear localization signal (nls) fused to the beta-galactosidase (lacZ) composite gene driven by the type 1 inositol 1,4,5-trisphosphate receptor (IP(3)R1) gene promoter. IP(3)R1-nls-lacZ transgene expression was detected at a single Purkinje cell level over the surface of a whole-mount X-gal-stained cerebellum because of nuclear accumulation of the nls-lacZ activity. Developing cerebella of 1NM13 mice showed stripe-like X-gal staining patterns of parasagittal Purkinje cell subsets. The X-gal stripe pattern was likely determined by an intrinsic property as early as E15 and showed increasing complexity with cerebellar development. The X-gal stripe pattern was reminiscent of, but not identical to, the stripe pattern of zebrin II immunoreactivity. We designated the symmetrical X-gal-positive (transgene-positive, Tg(+)) Purkinje cell stripes about the midline as vermal Tg1(+), Tg2(a, b)(+) and Tg3(a, b)(+) stripes and hemispheric Tg4...Continue Reading

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Citations

Jan 22, 2013·The Cerebellum·Izumi Sugihara, Hirofumi Fujita
May 21, 2014·ELife·Haibo ZhouMartijn Schonewille
Jul 22, 2014·Journal of Neurochemistry·Philip W BeesleyConstanze Seidenbecher
Mar 20, 2014·Biochimica Et Biophysica Acta·Hristina IvanovaGeert Bultynck
Apr 16, 2014·Frontiers in Systems Neuroscience·Richard Hawkes
Jan 1, 2020·The Journal of Comparative Neurology·Saddam K PanezaiIzumi Sugihara

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