PMID: 12770381May 29, 2003Paper

Expression of the juvenile hormone esterase gene in the Colorado potato beetle, Leptinotarsa decemlineata: photoperiodic and juvenile hormone analog response

Journal of Insect Physiology
A M VermuntC A de Kort

Abstract

Metamorphosis and reproduction in insects are controlled by juvenile hormone (JH). One of the factors, which regulate the JH titer in the hemolymph, is the activity of juvenile hormone esterase (JHE). JHE from the Colorado potato beetle, Leptinotarsa decemlineata, consists of two 57kDa subunits. In this study, the JHE-cDNA was used as a probe to examine where and when the gene is transcribed as well as how gene expression responds to photoperiodic treatment and to topical application with a JH analog, pyriproxyfen. JHE transcripts were almost exclusively found in RNA extracts from fat body tissue in both larvae and adults. JHE-mRNA levels in the fat body correlated positively with levels of JHE activity in the hemolymph. In the last larval instar, high levels of JHE-mRNA were found in the feeding stage. In adults, reared under short-day conditions, JHE-mRNA levels were high between day 2 and day 9, which correlated with high JHE activity in the hemolymph. During these conditions, the JH titer decreases in preparation for pupation and diapause, respectively. The JHE-mRNA levels and JHE activity in the hemolymph were higher in short-day than in reproductive long-day adults. If the JH analog pyriproxyfen was applied to animals of ...Continue Reading

References

Oct 1, 1977·Analytical Biochemistry·B D Hammock, T C Sparks
May 1, 1990·Archives of Biochemistry and Biophysics·V J WroblewskiB D Hammock
Mar 5, 1983·Journal of Molecular Biology·J G FilesD Hirsh
Jan 1, 1994·Developmental Genetics·V VenkataramanG Jones
Mar 21, 1998·Insect Biochemistry and Molecular Biology·A M VermuntC A de Kort

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Citations

Jun 16, 2007·Archives of Insect Biochemistry and Physiology·Manabu KamimuraMakoto Kiuchi
Aug 23, 2006·Photochemistry and Photobiology·Sakiko Shiga, Hideharu Numata
Jan 27, 2019·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Hong-Yue MaBao-Ping Pang
Oct 4, 2020·Insect Biochemistry and Molecular Biology·Gözde GüneyZekiye Suludere
Dec 8, 2005·Journal of Insect Physiology·Vladimír Kostál

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