Expression of the multiple antibiotic resistance operon (mar) during growth of Escherichia coli as a biofilm

Journal of Applied Microbiology
T Maira-LitránP Gilbert

Abstract

The multiple antibiotic resistance (mar) operon is a global regulator controlling the expression of various genes in Escherichia coli which constitutes the mar regulon. Upregulation of mar leads to a multi-drug resistant phenotype, which includes resistance towards structurally unrelated antibiotics, organic solvents and the disinfectant pine oil. Biofilms also display similar decreases in susceptibility to antimicrobial agents. A marOII-lacZ fusion strain (SPC105) of E. coli was used to monitor mar expression under various growth conditions including batch, continuous and biofilm culture. In chemically-defined media (CDM), mar expression was maximal in mid-log and declined in the stationary phase. Conversely, in rich media (Luria-Bertani broth), minimal expression in mid-log was followed by an increase in the stationary phase. In continuous culture, expression was inversely related to specific growth rate (mu = 0.05-0.4 h-1). LacZ expression by the marOII-lacZ fusion was generally low within the total biofilm population and equivalent to that of stationary phase cultures grown in batch culture. When the expression of mar in CDM batch culture was compared with that in biofilm populations, beta-galactosidase activity was general...Continue Reading

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Citations

Feb 13, 2001·Trends in Microbiology·T F Mah, G A O'Toole
Jan 16, 2003·Trends in Microbiology·L Julia Douglas
Oct 6, 2001·Current Opinion in Microbiology·K Poole
Dec 17, 2002·Symposium Series·K Poole
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Nov 21, 2012·Journal of Virology·Huahao FanYigang Tong
Jul 21, 2001·Lancet·P S Stewart, J W Costerton
Jun 10, 2014·Iranian Red Crescent Medical Journal·Maryam HeidaryMahboobeh Madani
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Apr 26, 2016·Nature Communications·Abhishek TrivediAshwani Kumar
Feb 1, 2015·International Journal of Antimicrobial Agents·Seiji YamasakiKunihiko Nishino
Oct 28, 2018·Journal of Biomedical Materials Research. Part a·Jazmín I González OcampoThomas J Webster
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Nov 1, 2005·EcoSal Plus·Christophe BeloinJean-Marc Ghigo

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