Expression of the NMDA receptor subunit GluN3A (NR3A) in the olfactory system and its regulatory role on olfaction in the adult mouse

Brain Structure & Function
Jin Hwan LeeShan Ping Yu

Abstract

Glutamate is an excitatory neurotransmitter in the olfactory system and its N-methyl-D-aspartate-(NMDA) receptor subunits [GluN1 (NR1), GluN2A (NR2A), and GluN2B (NR2B)] are expressed at synapses in the olfactory bulb and olfactory epithelium. Thus, glutamatergic neurons and NMDA receptors play key roles in olfaction. GluN3A (NR3A) is a unique inhibitory subunit in the NMDA receptor complex; however, the expression and functional role of GluN3A in the olfactory bulb and epithelium remain unclear. The present study examined the expression patterns of GluN3A in the olfactory bulb and epithelium and explored its functional role in the olfactory system. Immunohistochemical and Western blot analyses revealed that GluN3A is abundantly expressed in different cellular layers of the olfactory bulb and epithelium of the adult wild type (WT) mice. In littermate GluN3A knockout (GluN3A(-/-); KO) mice, the expression of olfactory marker protein normally found in mature olfactory sensory neurons was significantly reduced in the olfactory bulb and epithelium. A butyl alcohol stimulus increased immediate-early gene c-Fos expression in the olfactory system of WT mice, while this response was absent in GluN3A KO mice. The level of phosphorylated...Continue Reading

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Citations

Feb 23, 2020·Archivum Immunologiae Et Therapiae Experimentalis·Shenling LiNa Li
Dec 9, 2020·Cerebral Cortex·Alvaro MurilloIsabel Pérez-Otaño
May 5, 2021·The Journal of Physiology·Oliver CrawleyIsabel Pérez-Otaño
Jun 22, 2021·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Weiwei ZhongShan Ping Yu

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