Expression of the non-neuronal cholinergic system in human knee synovial tissue from patients with rheumatoid arthritis and osteoarthritis

Life Sciences
Jan SchubertKatrin S Lips

Abstract

As the stimulation of the α7-nicotinic acetylcholine receptor (nAChR), which is present in the synovium of patients with rheumatoid arthritis (RA), leads to a decrease in pro-inflammatory cytokines, the α7-nAChR is being discussed as a new therapeutic target. On this background we addressed the question whether α7-nAChR mRNA was differentially expressed in RA compared to osteoarthritis (OA) synovial samples and whether other components of the non-neuronal cholinergic system were also present and differentially expressed in the synovium of patients with RA in comparison to OA. The expression of nicotinic and muscarinic acetylcholine receptors (mAChRs), choline and acetylcholine transporters, synthesising and degrading enzymes was determined in human samples of synovial tissue from patients with RA and OA using RT-PCR and immunofluorescence labelling. Compared to OA, patients with RA showed increased expression of nAChR subunit β4 while a decline in subunits α2 and α4 as well as in mAChR M1R was observed. For all other nAChR subunits and mAChRs however there was no significant difference between RA and OA patients. With regard to the ACh transporters and enzymes no expressional changes were observed between OA and RA patients, ex...Continue Reading

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Citations

Jan 21, 2016·Arthritis Research & Therapy·Janet BeckmannKatrin Susanne Lips
Dec 8, 2014·Best Practice & Research. Clinical Rheumatology·F A KoopmanP P Tak
Apr 5, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Stephanie A Kleine, Steven C Budsberg
Jan 18, 2019·International Journal of Molecular Sciences·Katarzyna D SluzalskaJuergen Steinmeyer
Feb 21, 2021·Osteoarthritis and Cartilage·Marianne LauwersChunyi Wen

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