PMID: 9186949May 1, 1997Paper

Expression of the nonstructural protein NS1 of equine influenza A virus: detection of anti-NS1 antibody in post infection equine sera

Journal of Virological Methods
I Birch-MachinM Binns

Abstract

The nucleotide sequence of the nonstructural protein NS1 of the influenza virus A/equine 2/Suffolk/89 was determined and found to be 97% identical to that of A/equine 2/Miami/63. A similar level of identity was shown for the deduced NS1 amino acid sequence. The NS1 gene was expressed, in its entirety and in part, as fusion proteins with glutathione S-transferase using the pGEX-3X expression vector. Antibodies to NS1 protein were detected in serum samples from ponies experimentally infected with influenza virus, but not in animals vaccinated with whole inactivated virus or in unprimed control animals. The antigenic determinant(s) of NS1 protein appear to be located in the C-terminal half of the protein. The implications of these findings are discussed with reference to the use of NS1 protein as a differential diagnostic marker for influenza virus infection in the presence of high levels of circulating antibody to influenza haemagglutinin generated by recent vaccination.

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Citations

Aug 13, 2009·Archives of Virology·Ingrid KrejnusováGustáv Russ
Sep 16, 1999·Journal of Biotechnology·J T van Oirschot
Feb 24, 1999·Veterinary Microbiology·J T van OirschotP A van Rijn
Sep 17, 2005·Animal Health Research Reviews·Chang-Won Lee, David L Suarez
Aug 22, 2001·The Veterinary Quarterly·J T van Oirschot
Jul 4, 2002·The Veterinary Quarterly·C van Maanen, A Cullinane
Feb 14, 2013·Avian Diseases·David L Suarez
Oct 6, 2014·The Veterinary Clinics of North America. Equine Practice·Gabriele A Landolt
Feb 24, 2016·Viral Immunology·Noor Haliza HasanFarhid Hemmatzadeh
Feb 24, 2009·Veterinary Microbiology·Sílvia C BarrosMiguel Fevereiro
Nov 1, 2005·Biologicals : Journal of the International Association of Biological Standardization·David L Suarez
Dec 31, 2002·The Veterinary Journal·L A Babiuk
Feb 2, 2015·Current Opinion in Virology·Robert M Krug
Dec 1, 2009·Viruses·William G Dundon, Ilaria Capua

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