Expression of the oligomerization domain of the replication-associated protein (Rep) of Tomato leaf curl New Delhi virus interferes with DNA accumulation of heterologous geminiviruses

The Journal of Biological Chemistry
A ChatterjiC M Fauquet

Abstract

The minimal DNA binding domain of the replication-associated protein (Rep) of Tomato leaf curl New Delhi virus was determined by electrophoretic mobility gel shift analysis and co-purification assays. DNA binding activity maps to amino acids 1-160 (Rep-(1-160)) of the Rep protein and overlaps with the protein oligomerization domain. Transient expression of Rep protein (Rep-(1-160)) was found to inhibit homologous viral DNA accumulation by 70-86% in tobacco protoplasts and in Nicotiana benthamiana plants. The results obtained showed that expression of N-terminal sequences of Rep protein could efficiently interfere with DNA binding and oligomerization activities during virus infection. Surprisingly, this protein reduced accumulation of the African cassava mosaic virus, Pepper huasteco yellow vein virus and Potato yellow mosaic virus by 22-48%. electrophoretic mobility shift assays and co-purification studies showed that Rep-(1-160) did not bind with high affinity in vitro to the corresponding common region sequences of heterologous geminiviruses. However, Rep-(1-160) formed oligomers with the Rep proteins of the other geminiviruses. These data suggest that the regulation of virus accumulation may involve binding of the Rep to tar...Continue Reading

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Citations

Jan 5, 2005·Plant Molecular Biology·Padmanabhan ChellappanClaude M Fauquet
Aug 16, 2011·Virus Genes·Sukumaran SunithaKaruppannan Veluthambi
Jul 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ramachandran VanitharaniClaude M Fauquet
Feb 21, 2007·Plant Biotechnology Journal·Hervé VanderschurenPeng Zhang
Aug 29, 2014·PloS One·Dionne N ShepherdEdward P Rybicki
Aug 25, 2016·Molecular Plant Pathology·Syed Shan-E-Ali ZaidiShahid Mansoor

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