Expression of the orphan protein Plet-1 during trichilemmal differentiation of anagen hair follicles

The Journal of Investigative Dermatology
Karine RaymondArnoud Sonnenberg

Abstract

The rat mAb 33A10 recognizes an antigen in a variety of mouse epithelial tissues. In this study, we investigated in detail the expression pattern of the 33A10-defined antigen in the hair follicle. We show that 33A10 reactivity is confined to the most differentiated keratinocytes of the outer root sheath (ORS), the companion layer (CL), and to cells of the sebaceous gland duct. In vitro, the 33A10-defined antigen is expressed in keratinocytes derived from the ORS and accumulates on induction of differentiation. Using microarray analysis and transient transfection approaches, we established that the 33A10-defined antigen is the orphan protein, Placenta-expressed transcript (Plet)-1. Biochemical data indicated that Plet-1 is a glycosylphosphatidylinositol-anchored glycoprotein with N-linked carbohydrates in addition to other posttranslational modifications. Although silencing of Plet-1 expression using stable RNA interference in ORS keratinocytes decreased cellular migration, it increased adhesion to collagens I and IV. Immunohistochemical analysis showed that Plet-1 was primarily localized at the leading edge of epidermal wounds, where keratinocytes contacted the eschar. The restricted localization in both differentiated ORS and ...Continue Reading

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Citations

Oct 30, 2015·International Journal of Molecular Sciences·Nkemcho OjehOlivera Stojadinovic
Mar 22, 2012·The EMBO Journal·Benjamin Beck, Cédric Blanpain
Mar 22, 2016·Cell Reports·Svetlana UlyanchenkoC Clare Blackburn
Jan 10, 2012·Developmental Biology·Daniela Frances, Catherin Niemann
Jul 28, 2016·Stem Cell Research·Anders Patrik GunnarssonUffe Birk Jensen
Dec 13, 2016·International Journal of Molecular Sciences·Liu TengMei Yu
Oct 27, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jarod A ZeppXiaoxia Li
Dec 12, 2018·European Journal of Immunology·Julien J KarrichTom Cupedo
Aug 26, 2018·Scientific Reports·Michaela Zorn-KruppaJohanna M Brandner
May 19, 2019·Breast Cancer Research : BCR·Veronika RamovsArnoud Sonnenberg
Dec 30, 2018·The American Journal of Pathology·Sukalp MuzumdarMatthias Schäfer
Jul 14, 2021·Nature Methods·Nathanael AndrewsMartin Enge

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