Expression of the pho regulon interferes with induction of the uhpT gene in Escherichia coli K-12

Archives of Microbiology
S M HofferJ Tommassen

Abstract

The uptake of hexose 6-phosphates in Escherichia coli is mediated by the transporter UhpT, the synthesis of which is induced by the presence of glucose 6-phosphate (glucose 6-P) in the medium. Since this protein functions as an anion exchanger, it is generally assumed to be geared for the use of sugar phosphates as a carbon source. However, the question was unresolved whether this transporter can also provide the cells with glucose 6-P as a phosphate source. It is demonstrated in this work that UhpT-mediated glucose 6-P uptake does not allow the cells to grow on glucose 6-P as phosphate source. Hence, the expression of UhpT under phosphate limitation would not be particularly advantageous and some form of interaction between the uhp system and the Pi-limitation-inducible pho regulon, the products of which are involved in phosphate acquisition, may be anticipated. Indeed, the use of an uhpT-lacZ fusion revealed that much higher concentrations of the inducer glucose 6-P were required to elevate uhpT transcription when the pho regulon was expressed. This interference was the result of degradation of glucose 6-P by one of the products of the pho regulon, the periplasmic enzyme alkaline phosphatase. The specific form of interaction ...Continue Reading

Citations

Feb 12, 2014·Current Opinion in Microbiology·Ake Västermark, Milton H Saier
Apr 13, 2017·FEMS Microbiology Reviews·Davide Roncarati, Vincenzo Scarlato
Jan 28, 2021·International Journal of Molecular Sciences·Juan Francisco Martín, Paloma Liras

Related Concepts

UhpT protein, E coli
Alkaline Phosphatase
Alkalescens-Dispar Group
Hexose Transporter
Orthophosphate
Receptor Down-Regulation
Gene Expression Regulation, Bacterial
Regulon
Glucose-6-Phosphate
Escherichia coli Proteins

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